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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-RPS6KA1 (Thr359/Ser363) Polyclonal antibody

Phospho-RPS6KA1 (Thr359/Ser363) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29225-1-AP

Synonyms

HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Phospho-RPS6KA1 (Thr359/Ser363), Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-RPS6KA1 (Thr359/Ser363) Polyclonal antibod (29225-1-AP)

    WB analysis using 29225-1-AP

    Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29225-1-AP (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:3000 incubated at 4℃ overnight.

  • Western Blot (WB) analysis of various lysates using Phospho-RPS6KA1 (Thr359/Ser363) Polyclonal antibod (29225-1-AP)

    WB analysis using 29225-1-AP

    Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29225-1-AP (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Tested Applications

Positive WB detected inλ phosphatase treated NIH/3T3 cells, PDGF treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29225-1-AP targets Phospho-RPS6KA1 (Thr359/Ser363) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivity pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6 kinase, 90kDa, polypeptide 1
Calculated molecular weight 735 aa, 83 kDa
Observed molecular weight83-90 kDa
GenBank accession numberBC014966
Gene symbol RPS6KA1
Gene ID (NCBI) 6195
RRIDAB_2918254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and 0.05%BSA.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322)

Protocols

Product Specific Protocols
WB protocol for Phospho-RPS6KA1 (Thr359/Ser363) antibody 29225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

JTCVS Open

Intramyocardial injection of hypoxia-conditioned extracellular vesicles modulates apoptotic signaling in chronically ischemic myocardium

Authors - Dwight D Harris
View Article