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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-RPS6KA1 (Thr359/Ser363) Recombinant antibody

Phospho-RPS6KA1 (Thr359/Ser363) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3O10

Cat no : 81475-1-RR

Synonyms

HU 1, MAPKAP kinase 1a, MAPKAPK 1a, MAPKAPK1A, p90 RSK 1, p90RSK1, p90S6K, Phospho-RPS6KA1 (Thr359/Ser363), Ribosomal S6 kinase 1, RPS6KA1, RSK, RSK 1, RSK1, S6K alpha 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Phospho-RPS6KA1 (Thr359/Ser363) Recombinant antibo (81475-1-RR)

    WB analysis of HeLa using 81475-1-RR

    Non-treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 81475-1-RR (Phospho-RPS6KA1 (Thr359/Ser363) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta-Tubulin (66009-1-Ig) as loading control.

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Tested Applications

Positive WB detected inHeLa cells, λ phosphatase treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81475-1-RR targets Phospho-RPS6KA1 (Thr359/Ser363) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6 kinase, 90kDa, polypeptide 1
Calculated molecular weight 735 aa, 83 kDa
Observed molecular weight83-90 kDa
GenBank accession numberBC014966
Gene symbol RPS6KA1
Gene ID (NCBI) 6195
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322)

Protocols

Product Specific Protocols
WB protocol for Phospho-RPS6KA1 (Thr359/Ser363) antibody 81475-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols