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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-S6 Ribosomal protein (Ser235/236) Polyclonal antibody, PBS Only

Phospho-S6 Ribosomal protein (Ser235/236) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29223-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser235/236) Polyclon (29223-1-AP)

    WB analysis using 29223-1-AP (same clone as 29223-1-PBS)

    Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29223-1-AP (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 29223-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser235/236) Polyclon (29223-1-AP)

    WB analysis using 29223-1-AP (same clone as 29223-1-PBS)

    Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29223-1-AP (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29223-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29223-1-PBS targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6
Calculated molecular weight 29 kDa
Observed molecular weight32 kDa
GenBank accession numberBC000524
Gene symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.