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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser235) Monoclonal a (67898-1-Ig)

    WB analysis using 67898-1-Ig (same clone as 67898-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67898-1-Ig (Phospho-S6 Ribosomal protein (Ser235) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta tubulin antibody as loading control. This data was developed using the same antibody clone with 67898-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser235) Monoclonal a (67898-1-Ig)

    WB analysis using 67898-1-Ig (same clone as 67898-1-PBS)

    Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 67898-1-Ig (Phospho-S6 Ribosomal protein (Ser235) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta tubulin antibody as loading control. This data was developed using the same antibody clone with 67898-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser235) Monoclonal a (67898-1-Ig)

    WB analysis using 67898-1-Ig (same clone as 67898-1-PBS)

    Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 67898-1-Ig (Phospho-S6 Ribosomal protein (Ser235) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta tubulin antibody as loading control. This data was developed using the same antibody clone with 67898-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-S6 Ribosomal protein (Ser235) Monoclonal a (67898-1-Ig)

    IF Staining of HeLa using 67898-1-Ig (same clone as 67898-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed untreated and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-S6 Ribosomal protein (Ser235) antibody (67898-1-Ig, Clone: 2A4B6) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67898-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-S6 Ribosomal protein (Ser235) Monoclonal a (67898-1-Ig)

    FC experiment of HeLa using 67898-1-Ig (same clone as 67898-1-PBS)

    1x10^6 untreated HeLa cells (dash lines) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.2 μg Phospho-S6 Ribosomal protein (Ser235) Monoclonal antibody (67898-1-Ig, Clone: 2A4B6, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 67898-1-PBS in a different storage buffer formulation.

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Phospho-S6 Ribosomal protein (Ser235) Monoclonal antibody, PBS Only

Phospho-S6 Ribosomal protein (Ser235) Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67898-1-PBS
Clone No.2A4B6

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67898-1-PBS targets Phospho-S6 Ribosomal protein (Ser235) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6
Calculated molecular weight 29 kDa
Observed molecular weight32 kDa
GenBank accession numberBC000524
Gene Symbol RPS6
Gene ID (NCBI) 6194
RRIDAB_2918654
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62753
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404). 67898-1-Ig specifically recognizes the phosphorylation site of Ser235 or dual site Ser235/236.