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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-S6 Ribosomal protein (Ser235) Monoclonal antibody, PBS Only

Phospho-S6 Ribosomal protein (Ser235) Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A4B6

Cat no : 67898-1-PBS

Synonyms

40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, Phospho-RPS6 (Ser235), Phospho-S6 Ribosomal protein (Ser235), ribosomal protein S6, Ribosome Marker, RPS6, S6 ribosomal protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser235) Monoclonal a (67898-1-Ig)

    WB analysis using 67898-1-Ig (same clone as 67898-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67898-1-Ig (Phospho-S6 Ribosomal protein (Ser235) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta tubulin antibody as loading control. This data was developed using the same antibody clone with 67898-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser235) Monoclonal a (67898-1-Ig)

    WB analysis using 67898-1-Ig (same clone as 67898-1-PBS)

    Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 67898-1-Ig (Phospho-S6 Ribosomal protein (Ser235) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta tubulin antibody as loading control. This data was developed using the same antibody clone with 67898-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-S6 Ribosomal protein (Ser235) Monoclonal a (67898-1-Ig)

    WB analysis using 67898-1-Ig (same clone as 67898-1-PBS)

    Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 67898-1-Ig (Phospho-S6 Ribosomal protein (Ser235) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta tubulin antibody as loading control. This data was developed using the same antibody clone with 67898-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67898-1-PBS targets Phospho-S6 Ribosomal protein (Ser235) in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6
Calculated molecular weight 29 kDa
Observed molecular weight32 kDa
GenBank accession numberBC000524
Gene symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404). 67898-1-Ig specifically recognizes the phosphorylation site of Ser235 or dual site Ser235/236.