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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using Phospho-S6 Ribosomal protein (Ser240/244) Recombin (81184-1-RR)

    FC experiment of HeLa using 81184-1-RR (same clone as 81184-1-PBS)

    1x10^6 untreated HeLa cells (dash lines) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.4 μg Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody (81184-1-RR, Clone: 5J13, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR005). Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 81184-1-PBS in a different storage buffer formulation.

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Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody, PBS Only

Phospho-S6 Ribosomal protein (Ser240/244) Uni-rAbTM Recombinant Antibody for FC (Intra), Indirect ELISA
Cat No. 81184-1-PBS
Clone No.5J13

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), Indirect ELISA

Synonyms

p S6 Ribosomal protein Ser240/244, p S6 Ribosomal protein (Ser240/244), p S6 Ribosomal protein, Phospho S6 Ribosomal protein, Phospho S6 Ribosomal protein (Ser240/244)

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81184-1-PBS targets Phospho-S6 Ribosomal protein (Ser240/244) in FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6
Observed molecular weight32 kDa
GenBank accession numberNM_001010
Gene Symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62753
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ribosomal protein S6 (RPS6), also named Phosphoprotein NP33, may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens and is dephosphorylated at growth arrest.