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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody, PBS Only

Phospho-S6 Ribosomal protein (Ser240/244) Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5J13

Cat no : 81184-1-PBS

Synonyms

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Phospho-S6 Ribosomal protein (Ser240/244) Recombin (81184-1-RR)

    WB analysis of HeLa using 81184-1-RR (same clone as 81184-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 81184-1-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control. This data was developed using the same antibody clone with 81184-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Phospho-S6 Ribosomal protein (Ser240/244) Recombin (81184-1-RR)

    WB analysis of HEK-293 using 81184-1-RR (same clone as 81184-1-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81184-1-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control. This data was developed using the same antibody clone with 81184-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-S6 Ribosomal protein (Ser240/244) Recombin (81184-1-RR)

    IF Staining of HeLa using 81184-1-RR (same clone as 81184-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed untreated and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-S6 Ribosomal protein (Ser240/244) antibody (81184-1-RR, Clone: 5J13) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR004). This data was developed using the same antibody clone with 81184-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-S6 Ribosomal protein (Ser240/244) Recombin (81184-1-RR)

    FC experiment of HeLa using 81184-1-RR (same clone as 81184-1-PBS)

    1x10^6 untreated HeLa cells (dash lines) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.4 μg Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody (81184-1-RR, Clone: 5J13, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR005). Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 81184-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using Phospho-S6 Ribosomal protein (Ser240/244) Recombin (81184-1-RR)

    FC experiment of HEK-293 using 81184-1-RR (same clone as 81184-1-PBS)

    1x10^6 Calyculin A treated HEK-293 cells were intracellularly stained with 0.06 ug Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody (81184-1-RR, Clone:5J13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81184-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81184-1-PBS targets Phospho-S6 Ribosomal protein (Ser240/244) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6
Observed molecular weight32 kDa
GenBank accession numberNM_001010
Gene symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ribosomal protein S6 (RPS6), also named Phosphoprotein NP33, may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens and is dephosphorylated at growth arrest.