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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-SMAD1 (Ser195) Recombinant antibody

Phospho-SMAD1 (Ser195) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1K6

Cat no : 80257-1-RR

Synonyms

SMAD1, JV4-1, JV41, hSMAD1, BSP-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-SMAD1 (Ser195) Recombinant antibody (80257-1-RR)

    WB analysis using 80257-1-RR

    Non-treated and UV treated A431 cells were subjected to SDS PAGE followed by western blot with 80257-1-RR (Phospho-SMAD1 (Ser195) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Phospho-SMAD1 (Ser195) Recombinant antibody (80257-1-RR)

    WB analysis using 80257-1-RR

    Non-treated and BMP2 (HZ-1128) treated HepG2 cells were subjected to SDS PAGE followed by western blot with 80257-1-RR (Phospho-SMAD1 (Ser195) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA431 cells, BMP2 treated HepG2 cells, UV treated A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80257-1-RR targets Phospho-SMAD1 (Ser195) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SMAD family member 1
Calculated molecular weight 52 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC001878
Gene symbol SMAD1
Gene ID (NCBI) 4086
RRIDAB_2918881
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMAD family member 1 (Smad1) have been involved in metastatic progression of many cancer types. Smad1 can be induced by many tumor-stimulating cytokines such as the bone morphogenetic protein 2 (BMP2) and TNFα and plays important roles in cell invasion and metastasis. BMP2 signalling is initiated by binding to its specific receptors, which leads to in the phosphorylation and nuclear translocation of Smad1. Translocated Smad1 then modulates the expression of downstream osteogenic genes. (PMID: 32954678, PMID: 30008908)

Protocols

Product Specific Protocols
WB protocol for Phospho-SMAD1 (Ser195) antibody 80257-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols