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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-SMAD1 (Ser214) Polyclonal antibody (28895-1-AP)

    WB analysis using 28895-1-AP

    Non-treated HepG2 and BMP2 (HZ-1128) treated HepG2 cells were subjected to SDS PAGE followed by western blot with 28895-1-AP (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1 hours.

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Phospho-SMAD1 (Ser214) Polyclonal antibody

Phospho-SMAD1 (Ser214) Polyclonal Antibody for WB, ELISA
Cat No. 28895-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Synonyms

BSP 1, BSP1, hSMAD1, JV4 1, JV41, MAD homolog 1, Mad related protein 1, MADH1, MADR1, Mothers against DPP homolog 1, Phospho-SMAD1 (Ser214), SMAD 1, SMAD family member 1, SMAD1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBMP2 treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28895-1-AP targets Phospho-SMAD1 (Ser214) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SMAD family member 1
Calculated molecular weight 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC001878
Gene Symbol SMAD1
Gene ID (NCBI) 4086
RRIDAB_2918212
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15797
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMAD family member 1 (Smad1) have been involved in metastatic progression of many cancer types. Smad1 can be induced by many tumor-stimulating cytokines such as the bone morphogenetic protein 2 (BMP2) and TNFα and plays important roles in cell invasion and metastasis. BMP2 signalling is initiated by binding to its specific receptors, which leads to in the phosphorylation and nuclear translocation of Smad1. Translocated Smad1 then modulates the expression of downstream osteogenic genes. (PMID: 32954678, PMID: 30008908)

Protocols

Product Specific Protocols
WB protocol for Phospho-SMAD1 (Ser214) antibody 28895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Carbohydr Polym

In vitro and in silico anti-osteoporosis activities and underlying mechanisms of a fructan, ABW90-1, from Achyranthes bidentate.

Authors - Tianyu Li
View Article