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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Phospho-SMAD1 (Ser214) Recombinant antibody (81433-1-RR)

    WB analysis of A431 using 81433-1-RR (same clone as 81433-1-PBS)

    Non-treated and UV treated A431 cells were subjected to SDS PAGE followed by western blot with 81433-1-RR (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81433-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Phospho-SMAD1 (Ser214) Recombinant antibody (81433-1-RR)

    WB analysis of HepG2 using 81433-1-RR (same clone as 81433-1-PBS)

    Non-treated and Calyculin A treated HepG2 cells were subjected to SDS PAGE followed by western blot with 81433-1-RR (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81433-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-SMAD1 (Ser214) Recombinant antibody (81433-1-RR)

    WB analysis of NIH/3T3 using 81433-1-RR (same clone as 81433-1-PBS)

    Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81433-1-RR (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 81433-1-PBS in a different storage buffer formulation.

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Phospho-SMAD1 (Ser214) Recombinant antibody, PBS Only

Phospho-SMAD1 (Ser214) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 81433-1-PBS
Clone No.3K8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

SMAD1, JV4-1, JV41, hSMAD1, BSP-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81433-1-PBS targets Phospho-SMAD1 (Ser214) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SMAD family member 1
Calculated molecular weight 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC001878
Gene Symbol SMAD1
Gene ID (NCBI) 4086
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15797
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SMAD family member 1 (Smad1) have been involved in metastatic progression of many cancer types. Smad1 can be induced by many tumor-stimulating cytokines such as the bone morphogenetic protein 2 (BMP2) and TNFα and plays important roles in cell invasion and metastasis. BMP2 signalling is initiated by binding to its specific receptors, which leads to in the phosphorylation and nuclear translocation of Smad1. Translocated Smad1 then modulates the expression of downstream osteogenic genes. (PMID: 32954678, PMID: 30008908)