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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-SMAD9 (Ser465/467) Recombinant antibody

Phospho-SMAD9 (Ser465/467) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240470A12

Cat no : 80144-2-RR

Synonyms

p SMAD9, Pho SMAD9, Pho-SMAD9, Phospho SMAD9 (Ser465/467), Phospho SMAD9 Ser465/467

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Phospho-SMAD9 (Ser465/467) Recombinant antibody (80144-2-RR)

    WB analysis of HeLa using 80144-2-RR

    BMP2 treated HeLa cells were subjected to SDS PAGE followed by western blot with 80144-2-RR (SMAD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inBMP2 treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80144-2-RR targets Phospho-SMAD9 (Ser465/467) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SMAD family member 9
Calculated molecular weight 430 aa, 49 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC011559
Gene symbol SMAD9
Gene ID (NCBI) 4093
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mothers against decapentaplegic homolog 9 also known as SMAD9, SMAD8, and MADH6, belongs to the SMAD family of proteins, which belong to the TGFβ superfamily of modulators. Like many other TGFβ family members, SMAD9 is involved in cell signalling. When a bone morphogenetic protein binds to a receptor (BMP type 1 receptor kinase) it causes SMAD9 to interact with SMAD anchor for receptor activation (SARA).The binding of ligands causes the phosphorylation of the SMAD9 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD9 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase. There are two isoforms of the protein.

Protocols

Product Specific Protocols
WB protocol for Phospho-SMAD9 (Ser465/467) antibody 80144-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols