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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-STAT1 (Ser727) Recombinant antibody (80683-7-RR)

    WB analysis using 80683-7-RR

    Non treated and IFN gamma treated HeLa cells were subjected to SDS PAGE followed by western blot with 80683-7-RR (Phospho-STAT1 (Ser727) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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Phospho-STAT1 (Ser727) Recombinant antibody

Phospho-STAT1 (Ser727) Recombinant Antibody for WB, ELISA
Cat No. 80683-7-RR
Clone No.240897D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

phospho-S727 STAT1, Phospho STAT1 (Ser727), Phospho STAT1, p Stat1 (S727), phospho-STAT1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inIFN gamma treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80683-7-RR targets Phospho-STAT1 (Ser727) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 1, 91kDa
Calculated molecular weight 83 kDa
Observed molecular weight84-91 kDa
GenBank accession numberBC002704
Gene Symbol STAT1
Gene ID (NCBI) 6772
RRIDAB_3670483
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP42224
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 activating phosphorylation at Ser727 is p38-dependant duringlipotoxic stress. MLK3 activation triggers a MAPK signaling cascade resulting in STAT1 Ser727 phosphorylation. The phosphorylation of STAT1 Ser727 increases its nuclear localization, and transcriptional activity. (PMID: 28262979).

Protocols

Product Specific Protocols
WB protocol for Phospho-STAT1 (Ser727) antibody 80683-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols