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Host
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-STAT1 (Tyr701) Polyclonal antibody, PBS Only

Phospho-STAT1 (Tyr701) Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 28979-1-PBS

Synonyms

STAT1, Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-STAT1 (Tyr701) Polyclonal antibody (28979-1-AP)

    WB analysis using 28979-1-AP (same clone as 28979-1-PBS)

    Non-treated THP-1 cells, IFN gamma and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 28979-1-AP (Phospho-STAT1 (Tyr701) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28979-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-STAT1 (Tyr701) Polyclonal antibody (28979-1-AP)

    IF Staining of HeLa using 28979-1-AP (same clone as 28979-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using Phospho-STAT1 (Tyr701) antibody (28979-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28979-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of THP-1 cells using Phospho-STAT1 (Tyr701) Polyclonal antibody (28979-1-AP)

    FC experiment of THP-1 using 28979-1-AP (same clone as 28979-1-PBS)

    1x10^6 IFN gamma treated THP-1 cells were intracellularly stained with 0.25 ug Phospho-STAT1 (Tyr701) Polyclonal antibody (28979-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 28979-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28979-1-PBS targets Phospho-STAT1 (Tyr701) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 1, 91kDa
Calculated molecular weight 83 kDa
Observed molecular weight84-91 kDa
GenBank accession numberBC002704
Gene symbol STAT1
Gene ID (NCBI) 6772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).