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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-STAT1 (Tyr701) Recombinant antibody, PBS Only

Phospho-STAT1 (Tyr701) Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C5

Cat no : 82674-10-PBS

Synonyms

STAT1, Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using Phospho-STAT1 (Tyr701) Recombinant antibody (82674-10-RR)

    WB analysis of THP-1 using 82674-10-RR (same clone as 82674-10-PBS)

    Non-treated THP-1 cells and IFN gamma treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82674-10-RR (Phospho-STAT1 (Tyr701) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 82674-10-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of THP-1 cells using Phospho-STAT1 (Tyr701) Recombinant antibody (82674-10-RR)

    FC experiment of THP-1 using 82674-10-RR (same clone as 82674-10-PBS)

    1x10^6 IFN gamma treated THP-1 cells were intracellularly stained with 0.13 ug Phospho-STAT1 (Tyr701) Recombinant antibody (82674-10-RR, Clone:1C5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 82674-10-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82674-10-PBS targets Phospho-STAT1 (Tyr701) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 1, 91kDa
Calculated molecular weight 83 kDa
Observed molecular weight84-91 kDa
GenBank accession numberBC002704
Gene symbol STAT1
Gene ID (NCBI) 6772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).