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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig)

    WB analysis using 60479-1-Ig

    UV treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig)

    WB analysis using 60479-1-Ig

    EGF treated and untreated A549 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig)

    WB analysis using 60479-1-Ig

    TNF alpha (HZ-1014) treated and untreated MCF-7 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig)

    WB analysis using 60479-1-Ig

    TNF alpha (HZ-1014) treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control.

  • Western Blot (WB) analysis of HSC-T6 cells using Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig)

    WB analysis of HSC-T6 using 60479-1-Ig

    UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (STAT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig)

    IF Staining of HeLa using 60479-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Phospho-STAT3 (Ser727) antibody (60479-1-Ig, Clone: 2G11G11 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig)

    IF Staining of HeLa using 60479-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed untreated HeLa cells, UV (1 hour) and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-STAT3 (Ser727) antibody (60479-1-Ig, Clone: 2G11G11) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig)

    FC experiment of HeLa using 60479-1-Ig

    1x10^6 untreated HeLa cells (dash lines), UV (1 hour) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.1 μg Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig, Clone: 2G11G11, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2, blue) was parallel stained as control. Cells were fixed with 4% PFA .

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Phospho-STAT3 (Ser727) Monoclonal antibody

Phospho-STAT3 (Ser727) Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 60479-1-Ig
Clone No.2G11G11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

p STAT3 (Ser727), p STAT3, p STAT3 Ser727, Phospho STAT3, Phospho STAT3 (Ser727)

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A549 cells, UV treated HSC-T6 cells, MCF-7 cells, NIH/3T3 cells, EGF treated A549 cells, UV treated HeLa cells, TNF alpha treated MCF-7 cells
Positive IF/ICC detected inUV(1hour),100 nM Calyculin A(30 minutes)treated HeLa cells, HeLa cells
Positive FC (Intra) detected inUV (1 hour) and 100 nM Calyculin A (30 minutes) HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.10 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60479-1-Ig targets Phospho-STAT3 (Ser727) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Calculated molecular weight 770 aa, 88 kDa
Observed molecular weight85-90 kDa
GenBank accession numberBC000627
Gene Symbol STAT3
Gene ID (NCBI) 6774
RRIDAB_3670262
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP40763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.

Protocols

Product Specific Protocols
WB protocol for Phospho-STAT3 (Ser727) antibody 60479-1-IgDownload protocol
IF protocol for Phospho-STAT3 (Ser727) antibody 60479-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols