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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-STAT3 (Ser727) Monoclonal antibody

Phospho-STAT3 (Ser727) Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G11G11

Cat no : 60479-1-Ig

Synonyms

STAT3, STAT 3, HIES, APRF, Acute-phase response factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig)

    WB analysis using 60479-1-Ig

    UV treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig)

    WB analysis using 60479-1-Ig

    EGF treated and untreated A549 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig)

    WB analysis using 60479-1-Ig

    TNF alpha treated and untreated MCF-7 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig)

    WB analysis using 60479-1-Ig

    TNF alpha treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig)

    WB analysis of HSC-T6 using 60479-1-Ig

    UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (STAT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, UV treated HSC-T6 cells, MCF-7 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60479-1-Ig targets Phospho-STAT3 (Ser727) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Calculated molecular weight 770 aa, 88 kDa
Observed molecular weight85-90 kDa
GenBank accession numberBC000627
Gene symbol STAT3
Gene ID (NCBI) 6774
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.

Protocols

Product Specific Protocols
WB protocol for Phospho-STAT3 (Ser727) antibody 60479-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols