Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Polyclonal antibody
Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Polyclonal Antibody for WB, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 28949-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | GM-CSF treated TF-1 cells |
Positive FC (Intra) detected in | GM-CSF treated TF-1 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
28949-1-AP targets Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | signal transducer and activator of transcription 5A |
Calculated molecular weight | 794 aa, 92 kDa |
Observed molecular weight | 92-100 kDa |
GenBank accession number | BC027036 |
Gene symbol | STAT5A |
Gene ID (NCBI) | 6776 |
RRID | AB_2918219 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody 28949-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |