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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Polyclonal antibody

Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 28949-1-AP

Synonyms

STAT5A, STAT5, MGF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Polyclonal (28949-1-AP)

    WB analysis using 28949-1-AP

    Non-treated TF-1 and GM-CSF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 28949-1-AP (Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Flow cytometry (FC) experiment of TF-1 cells using Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Polyclonal (28949-1-AP)

    FC experiment of TF-1 using 28949-1-AP

    1x10^6 GM-CSF treated TF-1 cells were intracellularly stained with 0.13 ug Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Polyclonal antibody (28949-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

Positive WB detected inGM-CSF treated TF-1 cells
Positive FC (Intra) detected inGM-CSF treated TF-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28949-1-AP targets Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 5A
Calculated molecular weight 794 aa, 92 kDa
Observed molecular weight92-100 kDa
GenBank accession numberBC027036
Gene symbol STAT5A
Gene ID (NCBI) 6776
RRIDAB_2918219
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.

Protocols

Product Specific Protocols
WB protocol for Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody 28949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols