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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Monoclonal antibody

Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1F7G5

Cat no : 68000-1-Ig

Synonyms

STAT5A, STAT5, MGF, 1F7G5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Monoclonal (68000-1-Ig)

    WB analysis using 68000-1-Ig

    Non-treated and GM-CSF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 68000-1-lg (Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

  • Flow cytometry (FC) experiment of TF-1 cells using Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Monoclonal (68000-1-Ig)

    FC experiment of TF-1 using 68000-1-Ig

    1x10^6 GM-CSF treated TF-1 cells were intracellularly stained with 0.13 ug Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Monoclonal antibody (68000-1-Ig, Clone:1F7G5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.13 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

Positive WB detected inTF-1 cells, GM-CSF treated TF-1 cells
Positive FC (Intra) detected inGM-CSF treated TF-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68000-1-Ig targets Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 5A
Calculated molecular weight 794 aa, 92 kDa
Observed molecular weight90 kDa
GenBank accession numberBC027036
Gene symbol STAT5A
Gene ID (NCBI) 6776
RRIDAB_2918748
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human STAT5A.

Protocols

Product Specific Protocols
WB protocol for Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody 68000-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Rep

Copper (II) complex of salicylate phenanthroline induces the apoptosis of colorectal cancer cells, including oxaliplatin‑resistant cells

Authors - Zixin Liu
View Article