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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-TAK1 (Thr187) Recombinant antibody

Phospho-TAK1 (Thr187) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2O6

Cat no : 81785-1-RR

Synonyms

MAP3K7, Phospho-MAP3K7/TAK1 (Thr187), Phospho-TAK1 (Thr187), TAK1, TGF beta activated kinase 1, TGF1a

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Phospho-TAK1 (Thr187) Recombinant antibody (81785-1-RR)

    WB analysis of HEK-293 using 81785-1-RR

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81785-1-RR (Phospho-TAK1 (Thr187) antibody) at dilution of 1:100000 incubated at room temperature for 1 hour. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

  • Western Blot (WB) analysis of HeLa cells using Phospho-TAK1 (Thr187) Recombinant antibody (81785-1-RR)

    WB analysis of HeLa using 81785-1-RR

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 81785-1-RR (Phospho-TAK1 (Thr187) antibody) at dilution of 1:100000 incubated at room temperature for 1 hour. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

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Tested Applications

Positive WB detected inCalyculin A treated HEK-293 cells, Calyculin A treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81785-1-RR targets Phospho-TAK1 (Thr187) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase 7
Calculated molecular weight 579 aa, 64 kDa
Observed molecular weight75-85 kDa
GenBank accession numberBC017715
Gene symbol TAK1
Gene ID (NCBI) 6885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for Phospho-TAK1 (Thr187) antibody 81785-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols