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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-TBC1D1 (Ser660) Polyclonal antibody

Phospho-TBC1D1 (Ser660) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29034-1-AP

Synonyms

KIAA1108, Phospho-TBC1D1 (Ser660), TBC, TBC1, TBC1 domain family member 1, TBC1D1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-TBC1D1 (Ser660) Polyclonal antibody (29034-1-AP)

    WB analysis using 29034-1-AP

    Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 29034-1-AP (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1 hours.

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Tested Applications

Positive WB detected inCalyculin A treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29034-1-AP targets Phospho-TBC1D1 (Ser660) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
Calculated molecular weight 133 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC050321
Gene symbol TBC1D1
Gene ID (NCBI) 23216
RRIDAB_2918232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and 0.05% BSA
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

TBC1D1 belong to the family of RabGAPs and its physiological roles in glucose homeostasis and its implication in human diseases have already been characterized in skeletal muscle and adipocytes in a large number of independent studies. TBC1D1 including twophospho-tyrosine-binding (PTB) domains, a calmodulin-binding domain(CBD) and the func-tional GAP domain responsible for catalyzing GTP hydrolysis. TBC1D1 is phosphorylated by AMPK at Ser660 and Thr237, and by AKT at Thr596. The RabGAPs TBC1D1 is important regulators of glucose homeostasis inskeletal muscle and adipose tissue. (PMID: 31627187, PMID: 18477703)

Protocols

Product Specific Protocols
WB protocol for Phospho-TBC1D1 (Ser660) antibody 29034-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols