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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-TBC1D1 (Ser660) Recombinant antibody (81363-2-RR)

    WB analysis using 81363-2-RR (same clone as 81363-2-PBS)

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81363-2-RR (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. This data was developed using the same antibody clone with 81363-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-TBC1D1 (Ser660) Recombinant antibody (81363-2-RR)

    WB analysis using 81363-2-RR (same clone as 81363-2-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 81363-2-RR (Phospho-TBC1D1 (Ser660) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. This data was developed using the same antibody clone with 81363-2-PBS in a different storage buffer formulation.

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Phospho-TBC1D1 (Ser660) Recombinant antibody, PBS Only

Phospho-TBC1D1 (Ser660) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 81363-2-PBS
Clone No.7N19

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

TBC1D1, TBC1 domain family member 1, TBC1, KIAA1108, 7N19

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81363-2-PBS targets Phospho-TBC1D1 (Ser660) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
Calculated molecular weight 133 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC050321
Gene Symbol TBC1D1
Gene ID (NCBI) 23216
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86TI0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TBC1D1 belong to the family of RabGAPs and its physiological roles in glucose homeostasis and its implication in human diseases have already been characterized in skeletal muscle and adipocytes in a large number of independent studies. TBC1D1 including twophospho-tyrosine-binding (PTB) domains, a calmodulin-binding domain(CBD) and the func-tional GAP domain responsible for catalyzing GTP hydrolysis. TBC1D1 is phosphorylated by AMPK at Ser660 and Thr237, and by AKT at Thr596. The RabGAPs TBC1D1 is important regulators of glucose homeostasis inskeletal muscle and adipose tissue. (PMID: 31627187, PMID: 18477703)