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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-TBC1D1 (Ser707) Polyclonal antibody (29068-1-AP)

    WB analysis using 29068-1-AP

    Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 29068-1-AP (Phospho-TBC1D1 (Ser707) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as the loading control.

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Phospho-TBC1D1 (Ser707) Polyclonal antibody

Phospho-TBC1D1 (Ser707) Polyclonal Antibody for WB, ELISA
Cat No. 29068-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

KIAA1108, Phospho-TBC1D1 (Ser707), TBC, TBC1, TBC1 domain family member 1, TBC1D1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCalyculin A treated HEK-293T cells

This antibody is equivalent to Ser700 in mice.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29068-1-AP targets Phospho-TBC1D1 (Ser707) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
Calculated molecular weight 133 kDa
Observed molecular weight150-160 kDa
GenBank accession numberBC050321
Gene Symbol TBC1D1
Gene ID (NCBI) 23216
RRIDAB_3085341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86TI0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBC1D1 belong to the family of RabGAPs and its physiological roles in glucose homeostasis and its implication in human diseases have already been characterized in skeletal muscle and adipocytes in a large number of independent studies. TBC1D1 including twophospho-tyrosine-binding (PTB) domains, a calmodulin-binding domain(CBD) and the func-tional GAP domain responsible for catalyzing GTP hydrolysis. TBC1D1 is phosphorylated by AMPK at Ser660 and Thr237, and by AKT at Thr596. The RabGAPs TBC1D1 is important regulators of glucose homeostasis inskeletal muscle and adipose tissue. (PMID: 31627187, PMID: 18477703)

Protocols

Product Specific Protocols
WB protocol for Phospho-TBC1D1 (Ser707) antibody 29068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols