Phospho-TBK1 (Ser172) Recombinant antibody
Phospho-TBK1 (Ser172) Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (3)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2K2
Cat no : 82383-1-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Calyculin A treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
82383-1-RR targets Phospho-TBK1 (Ser172) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | TANK-binding kinase 1 |
| Observed molecular weight | 84 kDa |
| GenBank accession number | BC034950 |
| Gene symbol | TBK1 |
| Gene ID (NCBI) | 29110 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-TBK1 (Ser172) antibody 82383-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther The cGAS-STING pathway-dependent sensing of mitochondrial DNA mediates ocular surface inflammation | ||
Eur J Pharmacol Nitisinone attenuates cartilage degeneration and subchondral osteoclastogenesis in osteoarthritis and concomitantly inhibits the cGAS/STING/NF-κB pathway | ||
Cell Rep Non-canonical isoforms of the mRNA polyadenylation factor WDR33 regulate STING-mediated immune responses | ||
Br J Pharmacol Glycyrrhetinic acid blocks SARS-CoV-2 infection by activating the cGAS-STING signalling pathway | ||
J Virol Pseudorabies virus infection triggers mitophagy to dampen the interferon response and promote viral replication |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Marina (Verified Customer) (09-27-2023) | Western blot using p-TBK1 antibody at 1:5000 dilution overnight at 4ºC. Samples are two breast cancer (BC) cell lines, ran in duplicate. Ponceau staining is shown as total protein loading control. A more prominent band can be seen below 100 kDa, and after more exposure, another band at around 80 kDa can be observed.
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