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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-TDP43 (Ser409/410) Monoclonal antibody, PBS Only

Phospho-TDP43 (Ser409/410) Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A2C1

Cat no : 66318-1-PBS

Synonyms

TARDBP, TDP 43, TDP43, TDP-43, TAR DNA-binding protein 43

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Phospho-TDP43 (Ser409/410) Monoclonal antibody (66318-1-Ig)

    WB analysis of HeLa using 66318-1-Ig (same clone as 66318-1-PBS)

    Untreated and EA treated HeLa cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) Antibody) at dilution of 1:850 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Phospho-TDP43 (Ser409/410) Monoclonal antibody (66318-1-Ig)

    WB analysis of human placenta using 66318-1-Ig (same clone as 66318-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Phospho-TDP43 (Ser409/410) Monoclonal antibody (66318-1-Ig)

    WB analysis of HEK-293 using 66318-1-Ig (same clone as 66318-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using Phospho-TDP43 (Ser409/410) Monoclonal antibody (66318-1-Ig)

    WB analysis of C6 using 66318-1-Ig (same clone as 66318-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410)antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using Phospho-TDP43 (Ser409/410) Monoclonal antibody (66318-1-Ig)

    WB analysis of Neuro-2a using 66318-1-Ig (same clone as 66318-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66318-1-PBS targets Phospho-TDP43 (Ser409/410) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 45-50 kDa, 35 kDa
GenBank accession numberNM_007375
Gene symbol TDP-43
Gene ID (NCBI) 23435
RRIDAB_2881699
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 66318-1-Ig is a phospho-dependent monoclonal antibody specifically recognizing phospho-TDP43 (409/410). It does not react with native TDP-43.