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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-TSC2 (Ser939) Recombinant antibody (81654-1-RR)

    WB analysis using 81654-1-RR (same clone as 81654-1-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81654-1-RR (Phospho-TSC2 (Ser939) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (66888-1-Ig) as the loading control. This data was developed using the same antibody clone with 81654-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using Phospho-TSC2 (Ser939) Recombinant antibody (81654-1-RR)

    FC experiment of HEK-293 using 81654-1-RR (same clone as 81654-1-PBS)

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A were intracellularly stained with 0.06 ug Phospho-TSC2 (Ser939) Recombinant antibody (81654-1-RR, Clone:2B18) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81654-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81654-1-PBS targets Phospho-TSC2 (Ser939) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tuberous sclerosis 2
Calculated molecular weight 1807 aa, 201 kDa
Observed molecular weight200 kDa
GenBank accession numberBC150300
Gene Symbol TSC2
Gene ID (NCBI) 7249
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49815
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Tuberin is phosphorylated on Ser939 and Thr1462 in response to PI3K activation and the human TSC complex is a direct biochemical target of the PI3K/Akt pathway (PMID: 12150915).