• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-TSC2 (Thr1462) Polyclonal antibody

Phospho-TSC2 (Thr1462) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29000-1-AP

Synonyms

FLJ43106, LAM, Phospho-TSC2 (Thr1462), TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-TSC2 (Thr1462) Polyclonal antibody (29000-1-AP)

    WB analysis using 29000-1-AP

    Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29000-1-AP (Phospho-TSC2 (Thr1462) antibody) at dilution of 1:1000 incubated at 37℃ for 1 hour.

  • Western Blot (WB) analysis of various lysates using Phospho-TSC2 (Thr1462) Polyclonal antibody (29000-1-AP)

    WB analysis using 29000-1-AP

    Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29000-1-AP (Phospho-TSC2 (Thr1462) antibody) at dilution of 1:1000 incubated at 37℃ for 1 hour.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inPDGF treated PC-3 cells, PDGF treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

29000-1-AP targets Phospho-TSC2 (Thr1462) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tuberous sclerosis 2
Calculated molecular weight 1807 aa, 201 kDa
Observed molecular weight200 kDa
GenBank accession numberBC150300
Gene symbol TSC2
Gene ID (NCBI) 7249
RRIDAB_2918227
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and 0.05% BSA
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

TSC2, also named as Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It has been reported that TSC2 can be phosphorylated at Thr1462 by Akt/PKB involved in stimulating cell growth and is activated by insulin. (PMID: 14651849, 12172553)

Protocols

Product Specific Protocols
WB protocol for Phospho-TSC2 (Thr1462) antibody 29000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Research (Wash D C)

The FTO Mediated N6-Methyladenosine Modification of DDIT4 Regulation with Tumorigenesis and Metastasis in Prostate Cancer

Authors - Yue Zhao
View Article