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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-VPS34 (Thr159) Polyclonal antibody (29675-1-AP)

    WB analysis using 29675-1-AP

    Non-treated SH-SY5Y cells, phosphatase inhibitor treated SH-SY5Y cells cells and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29675-1-AP (Phospho-VPS34 (Thr159) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Phospho-VPS34 (Thr159) Polyclonal antibody

Phospho-VPS34 (Thr159) Polyclonal Antibody for WB, ELISA
Cat No. 29675-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, λ phosphatase treated SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29675-1-AP targets Phospho-VPS34 (Thr159) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, class 3
Calculated molecular weight102 kDa
Observed molecular weight150 kDa
GenBank accession numberNM_002647
Gene Symbol VPS34
Gene ID (NCBI) 5289
RRIDAB_3669693
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NEB9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS34 phosphorylates phosphatidylinositol to produce PtdIns3P and is the progenitor of the phosphoinositide 3-kinase (PI3K) family. VPS34 has a simpler domain organization than class I PI3Ks, which belies the complexity of its quaternary organization, with the enzyme always functioning within larger assemblies. (PMID: 30397185)

Protocols

Product Specific Protocols
WB protocol for Phospho-VPS34 (Thr159) antibody 29675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols