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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-c-MYC (Ser62) Polyclonal antibody (28915-1-AP)

    WB analysis using 28915-1-AP

    Non-treated and MG132 treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28915-1-AP (Phospho-c-MYC (Ser62) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Phospho-c-MYC (Ser62) Polyclonal antibody

Phospho-c-MYC (Ser62) Polyclonal Antibody for WB, ELISA
Cat No. 28915-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, Phopho-Ser62-MYC, Proto oncogene c Myc, Transcription factor p64

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMG132 treated HEK-293T cells, HEK-293T cells

This site is also equivalent to Ser77 for c-MYC isoform 2 (P01106-2).

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28915-1-AP targets Phospho-c-MYC (Ser62) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated molecular weight 49 kDa
Observed molecular weight55 kDa
GenBank accession numberBC000141
Gene Symbol MYC
Gene ID (NCBI) 4609
RRIDAB_3085340
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01106
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYC is a transcription factor that globally enhances expression of transcribing genes, including those vital for cell cycle, growth, proliferation, and survival in normal and cancer cells. MYC has multiple isomers, and this antibody recognizes the phosphorylation of the 439 amino acid isomer (P01106-1, UniProt) at the 62 serine site. Posttranslational modifications that regulate MYC stability include phosphorylations at Ser62 and Thr58. Phosphorylation at Ser62, primarily by ERK, stabilizes MYC. While kinases other than ERK phosphorylate Ser62, GSK3β has been the only kinase known to phosphorylate MYC at Thr58.(PMID: 32482868)

Protocols

Product Specific Protocols
WB protocol for Phospho-c-MYC (Ser62) antibody 28915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols