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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-eEF2K (Ser366) Polyclonal antibody

Phospho-eEF2K (Ser366) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29032-1-AP

Synonyms

eEF 2 kinase, eEF 2K, eEF2K, HSU93850, Phospho-eEF2K (Ser366)

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-eEF2K (Ser366) Polyclonal antibody (29032-1-AP)

    WB analysis using 29032-1-AP

    Non-treated and λ phosphatase treated Jurkat cells were subjected to SDS PAGE followed by western blot with 29032-1-AP (Phospho-eEF2K (Ser366) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inJurkat cells, λ phosphatase treated Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29032-1-AP targets Phospho-eEF2K (Ser366) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic elongation factor-2 kinase
Calculated molecular weight 725 aa, 100 kDa
Observed molecular weight100 kDa
GenBank accession numberBC032665
Gene symbol EEF2K
Gene ID (NCBI) 29904
RRIDAB_2918231
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic Elongation Factor-2 Kinase (eEF2K) acts as a negative regulator of protein synthesis, translation, and cell growth. As a structurally unique member of the alpha-kinase family, eEF2K is essential to cell survival under stressful conditions, as it contributes to both cell viability and proliferation. eEF2K is regulated by various mechanisms, including phosphorylation through residues and autophosphorylation. eEF2K is regulated by various mechanisms, including phosphorylation through residues and autophosphorylation. eEF2K is downregulated through the phosphorylation of multiple sites via mTOR signaling and upregulated via the AMPK pathway. In the S6K-mediated pathway, RPS6KB1 or p70S6K, can phosphorylate eEF2K on Ser-366, rendering it inactive(PMID: 11500364, PMID: 34532346). The calculated molecular weight of eEF2K is 82kDa, and probably due to phosphorylation modification, resulting in a larger molecular weight, around 100kDa.

Protocols

Product Specific Protocols
WB protocol for Phospho-eEF2K (Ser366) antibody 29032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols