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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-eEF2K (Thr57) Polyclonal antibody (30811-1-AP)

    WB analysis using 30811-1-AP

    Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 30811-1-AP (Phospho-eEF2K (Thr57) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-eEF2K (Thr57) Polyclonal antibody (30811-1-AP)

    WB analysis using 30811-1-AP

    Non-treated NIH/3T3 cells and Forskolin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 30811-1-AP (Phospho-eEF2K (Thr57) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Phospho-eEF2K (Thr57) Polyclonal antibody

Phospho-eEF2K (Thr57) Polyclonal Antibody for WB, ELISA
Cat No. 30811-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

eEF 2 kinase, eEF 2K, EEF2K, HSU93850, Phospho-eEF2K (Thr57)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, λ phosphatase treated HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30811-1-AP targets Phospho-eEF2K (Thr57) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name eukaryotic elongation factor-2 kinase
Calculated molecular weight 725 aa, 100 kDa
Observed molecular weight100 kDa
GenBank accession numberBC032665
Gene Symbol EEF2K
Gene ID (NCBI) 29904
RRIDAB_3669761
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00418
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic elongation factor‐2 kinase (eEF2K), well‐known to be a Ca2+ calmodulin (CaM)‐dependent kinase, is overexpressed in several types of malignancies, most notably Triple negative breast cancer (TNBC). Increasing evidence indicates that eEF2K may modulate the expression of some apoptotic proteins such as XIAP, c‐FLIPL, Bcl‐XL, PI3KCI and p70S6K to inhibit apoptotic process in cancer. On the other hand, it may regulate autophagy involved in AMPK, mTORC1 and ULK1, thereby promoting cancer cell survival. (PMID: 29094413)

Protocols

Product Specific Protocols
WB protocol for Phospho-eEF2K (Thr57) antibody 30811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols