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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-mTOR (Ser2448) Monoclonal antibody, PBS Only

Phospho-mTOR (Ser2448) Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2A12G3

Cat no : 67778-1-PBS

Synonyms

FRAP1, MTOR, FRAP2, FRAP, FKBP12-rapamycin complex-associated protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig)

    WB analysis using 67778-1-Ig (same clone as 67778-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig)

    WB analysis using 67778-1-Ig (same clone as 67778-1-PBS)

    Non-treated and EGF treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig)

    WB analysis using 67778-1-Ig (same clone as 67778-1-PBS)

    Non-treated and Rapamycin treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig)

    WB analysis using 67778-1-Ig (same clone as 67778-1-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig)

    WB analysis of HSC-T6 using 67778-1-Ig (same clone as 67778-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig)

    WB analysis using 67778-1-Ig (same clone as 67778-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig)

    IHC staining of human colon cancer using 67778-1-Ig (same clone as 67778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig)

    IHC staining of human breast cancer using 67778-1-Ig (same clone as 67778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig)

    IHC staining of human breast cancer using 67778-1-Ig (same clone as 67778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig)

    IHC staining of human colon cancer using 67778-1-Ig (same clone as 67778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig)

    IF Staining of HepG2 using 67778-1-Ig (same clone as 67778-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-mTOR (Ser2448) antibody (67778-1-Ig, Clone: 2A12G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig)

    FC experiment of HeLa using 67778-1-Ig (same clone as 67778-1-PBS)

    1x10^6 100 nM Calyculin A (30 minutes) treated HeLa cells were intracellularly stained with 0.2 μg Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig, Clone:2A12G3) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005), and 0.2 μg KLH (66360-3-Ig, Clone: K11B8C4B5). Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig)

    FC experiment of HeLa using 67778-1-Ig (same clone as 67778-1-PBS)

    1X10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.5 ug Anti-Human Phospho-mTOR (Ser2448) (67778-1-Ig, Clone:2A12G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67778-1-PBS targets Phospho-mTOR (Ser2448) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 12-rapamycin associated protein 1
Calculated molecular weight 289 kDa
Observed molecular weight 289 kDa
GenBank accession numberBC117166
Gene symbol mTOR
Gene ID (NCBI) 2475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.