Phospho-mTOR (Ser2481) Polyclonal antibody
Phospho-mTOR (Ser2481) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Mouse and More (2)
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 28879-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Insulin treated NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
Product Information
28879-1-AP targets Phospho-mTOR (Ser2481) in WB, ELISA applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | FK506 binding protein 12-rapamycin associated protein 1 |
| Calculated molecular weight | 289 kDa |
| Observed molecular weight | 250-289 kDa |
| GenBank accession number | BC117166 |
| Gene symbol | mTOR |
| Gene ID (NCBI) | 2475 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MTOR, also named FRAP1, FRAP, FRAP2, and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is the kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. mTORC2 is also activated by growth factors but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-mTOR (Ser2481) antibody 28879-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Iridoids from Gardeniae fructus ameliorates depression by enhancing synaptic plasticity via AMPA receptor-mTOR signaling | ||
Chem Biol Interact Oral administration of sophoricoside (SOP) inhibits neuronal damage and neuroinflammation to curb neurodegeneration in Parkinson's disease | ||
Biochem Pharmacol Targeting FTO suppresses hepatocellular carcinoma by inhibiting ERBB3 and TUBB4A expression | ||
Front Pharmacol Revealing the role of metformin in gastric intestinal metaplasia treatment | ||
Cell Rep Synergistic hyperactivation of both mTORC1 and mTORC2 underlies the neural abnormalities of PTEN-deficient human neurons and cortical organoids |
