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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-p130Cas / BCAR1 (Tyr410) Polyclonal antibo (30887-1-AP)

    WB analysis using 30887-1-AP

    Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 30887-1-AP (Phospho-p130Cas / BCAR1 (Tyr410) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with p130Cas / BCAR1 antibody (16815-1-AP) subsequently.

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Phospho-p130Cas / BCAR1 (Tyr410) Polyclonal antibody

Phospho-p130Cas / BCAR1 (Tyr410) Polyclonal Antibody for WB, ELISA
Cat No. 30887-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

BCAR1, CAS, CAS1, CASS1, CRK associated substrate, CRKAS, P130Cas

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inpervanadate treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30887-1-AP targets Phospho-p130Cas / BCAR1 (Tyr410) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name breast cancer anti-estrogen resistance 1
Calculated molecular weight 870 aa, 93 kDa
Observed molecular weight130 kDa
GenBank accession numberBC062556
Gene Symbol BCAR1
Gene ID (NCBI) 9564
RRIDAB_3669775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56945
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

p130Cas, encoded by the breast cancer anti-estrogen resistance 1 (BCAR1) gene, is a scaffold protein that integrates large multi-protein complexes in response to stimuli such as hormones, growth factors, and integrin engagement. Upon environmental signals, Src family members and focal adhesion kinase (FAK) phosphorylate the p130Cas substrate domain (SD). This active p130Cas can recruit adaptor proteins such as Crk and Nck to its phosphorylated SD. (PMID: 34830244)

Protocols

Product Specific Protocols
WB protocol for Phospho-p130Cas / BCAR1 (Tyr410) antibody 30887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols