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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal antibody, PBS Only

Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28796-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal antibo (28796-1-AP)

    WB analysis using 28796-1-AP (same clone as 28796-1-PBS)

    Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:2000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28796-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal antibo (28796-1-AP)

    WB analysis using 28796-1-AP (same clone as 28796-1-PBS)

    Non-treated and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28796-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal antibo (28796-1-AP)

    WB analysis using 28796-1-AP (same clone as 28796-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28796-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal antibo (28796-1-AP)

    IF Staining of HepG2 using 28796-1-AP (same clone as 28796-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-p38 MAPK (Thr180/Tyr182) antibody (28796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28796-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal antibo (28796-1-AP)

    IF Staining of THP-1 using 28796-1-AP (same clone as 28796-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LPS treated THP-1 cells using Phospho-p38 MAPK (Thr180/Tyr182) antibody (28796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28796-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28796-1-PBS targets Phospho-p38 MAPK (Thr180/Tyr182) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 14
Calculated molecular weight 360 aa, 41 kDa
Observed molecular weight38-42 kDa
GenBank accession numberBC031574
Gene symbol p38 MAPK
Gene ID (NCBI) 1432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

A stress-activated serine/threonine protein kinase, p38 mitogen-activated protein kinase (p38 MAPK), belongs to the MAP kinase superfamily. Diverse extracellular stimuli, including ultraviolet light, irradiation, heat shock, high osmotic stress, proinflammatory cytokines and certain mitogens, trigger a stress-regulated protein kinase cascade culminating in activation of p38 MAPK through phosphorylation on a TGY motif within the kinase activation loop. The p38 MAPK undergoes dual phosphorylation at Thr182 and Tyr180 in the Thr-Gly-Tyr activation loop by MAP kinase kinase 6 (MKK6). Upon activation, p38 MAPK phosphorylates multiple substrates, including MAPK activated protein kinase 2 (MAPKAPK2) and activating transcription factor 2 (ATF-2). (PMID: 26901653, PMID: 10807318)