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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-p70(S6K) (Thr421/Ser424) Polyclonal antibody

Phospho-p70(S6K) (Thr421/Ser424) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29248-1-AP

Synonyms

p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, P70S6K1, Phospho-p70(S6K) (Thr421/Ser424), PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-p70(S6K) (Thr421/Ser424) Polyclonal antibo (29248-1-AP)

    WB analysis using 29248-1-AP

    Non-treated MCF-7 cells, phosphatase inhibitor treated and λ phosphatase MCF-7 cells were subjected to SDS PAGE followed by western blot with 29248-1-AP (Phospho-p70(S6K) (Thr421/Ser424) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-p70(S6K) (Thr421/Ser424) Polyclonal antibo (29248-1-AP)

    IF Staining of HeLa using 29248-1-AP

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-p70(S6K) (Thr421/Ser424) antibody (29248-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inλ phosphatase treated MCF-7 cells
Positive IF/ICC detected inλ phosphatase treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29248-1-AP targets Phospho-p70(S6K) (Thr421/Ser424) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6 kinase, 70kDa, polypeptide 1
Calculated molecular weight 59 kDa
Observed molecular weight65-85 kDa
GenBank accession numberBC053365
Gene symbol p70 S6K
Gene ID (NCBI) 6198
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway. P70S6K plays a crucial role in controlling cell cycle, growth and survival. The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). In the conventional model, Thr421/Ser424 sites are phosphorylated before Thr389 phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) and subsequent Thr229 phosphorylation by PDK1. (PMID: 25100792, PMID: 24970012, PMID: 21602892)

Protocols

Product Specific Protocols
WB protocol for Phospho-p70(S6K) (Thr421/Ser424) antibody 29248-1-APDownload protocol
IF protocol for Phospho-p70(S6K) (Thr421/Ser424) antibody 29248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols