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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using Placental lactogen Monoclonal antibody (66728-1-Ig)

    WB analysis of human placenta using 66728-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66728-1-Ig (Placental lactogen antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Placental lactogen Monoclonal antibody (66728-1-Ig)

    IHC staining of human placenta using 66728-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66728-1-Ig (Placental lactogen antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Placental lactogen Monoclonal antibody (66728-1-Ig)

    IHC staining of human placenta using 66728-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66728-1-Ig (Placental lactogen antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Placental lactogen Monoclonal antibody

Placental lactogen Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66728-1-Ig
Clone No.1D11E4

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CSH2, CSH1, CSB, CS 2, Choriomammotropin

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66728-1-Ig targets Placental lactogen in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen Placental lactogen fusion protein Ag9412 相同性解析による交差性が予測される生物種
Full Name chorionic somatomammotropin hormone 2
Calculated molecular weight 217 aa, 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC022044
Gene Symbol CSH2
Gene ID (NCBI) 1443
RRIDAB_2882078
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01243
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human growth hormone (GH) locus is comprised by two GH (GH1 and GH2) genes and three chorionic somatomammotropin (CSH1, CSH2 and CSH-L) genes. Placental lactogens (CSH1, CSH2) are produced in the mammalian placenta and are similar in structure and function to growth hormones. Together, placental lactogens and growth factors play an essential role to assure successful lactation after pregnancy. Placental lactogens also modify the metabolic state of the mother during pregnancy to supply energy to the fetus. Both CSH1 and CSH2 can be detected by this antibody.

Protocols

Product Specific Protocols
WB protocol for Placental lactogen antibody 66728-1-IgDownload protocol
IHC protocol for Placental lactogen antibody 66728-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols