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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Plasminogen Polyclonal antibody (18300-1-AP)

    WB analysis using 18300-1-AP (same clone as 18300-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 18300-1-AP (Plasminogen antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18300-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Plasminogen Polyclonal antibody (18300-1-AP)

    WB analysis of HepG2 using 18300-1-AP (same clone as 18300-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18300-1-AP (Plasminogen antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18300-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Plasminogen Polyclonal antibody (18300-1-AP)

    IHC staining of human liver using 18300-1-AP (same clone as 18300-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18300-1-AP (Plasminogen antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18300-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Plasminogen Polyclonal antibody (18300-1-AP)

    IHC staining of human liver using 18300-1-AP (same clone as 18300-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18300-1-AP (Plasminogen antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18300-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Plasminogen Polyclonal antibody (18300-1-AP)

    IF Staining of HepG2 using 18300-1-AP (same clone as 18300-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18300-1-AP (Plasminogen antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18300-1-PBS in a different storage buffer formulation.

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Plasminogen Polyclonal antibody, PBS Only

Plasminogen Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18300-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18300-1-PBS targets Plasminogen in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name plasminogen
Calculated molecular weight 91 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC060513
Gene Symbol Plasminogen
Gene ID (NCBI) 5340
RRIDAB_2167096
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00747
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.