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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Plasminogen Polyclonal antibody

Plasminogen Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26768-1-AP

Synonyms

Angiostatin, plasminogen, PLG

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Plasminogen Polyclonal antibody (26768-1-AP)

    WB analysis of human plasma using 26768-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 26768-1-AP (Plasminogen antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using Plasminogen Polyclonal antibody (26768-1-AP)

    WB analysis of Raji using 26768-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 26768-1-AP (Plasminogen antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Plasminogen Polyclonal antibody (26768-1-AP)

    IHC staining of human liver cancer using 26768-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26768-1-AP (Plasminogen antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Plasminogen Polyclonal antibody (26768-1-AP)

    IHC staining of human liver cancer using 26768-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26768-1-AP (Plasminogen antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman plasma tissue, Raji cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26768-1-AP is Plasminogen Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Plasminogen fusion protein Ag25243 相同性解析による交差性が予測される生物種
Full Name plasminogen
Calculated molecular weight 810 aa, 91 kDa
Observed molecular weight 91 kDa
GenBank accession numberBC060513
Gene symbol Plasminogen
Gene ID (NCBI) 5340
RRIDAB_2880627
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.

Protocols

Product Specific Protocols
WB protocol for Plasminogen antibody 26768-1-APDownload protocol
IHC protocol for Plasminogen antibody 26768-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols