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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Podocalyxin Monoclonal antibody

Podocalyxin Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4A3C7

Cat no : 68250-1-Ig

Synonyms

GCTM 2 antigen, Gp200, PC, PCLP, PCLP 1, PCLP1, Podocalyxin, podocalyxin like, Podocalyxin like protein 1, PODXL, RCC marker, TRA 1 81,TRA 1 60

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human kidney tissue using Podocalyxin Monoclonal antibody (68250-1-Ig)

    IHC staining of human kidney using 68250-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68250-1-Ig (Podocalyxin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Podocalyxin Monoclonal antibody (68250-1-Ig)

    IHC staining of human kidney using 68250-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68250-1-Ig (Podocalyxin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using Podocalyxin Monoclonal antibody (68250-1-Ig)

    IF Staining of human kidney using 68250-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Podocalyxin antibody (68250-1-Ig, Clone: 4A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using Podocalyxin Monoclonal antibody (68250-1-Ig)

    IF Staining of human kidney using 68250-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Podocalyxin antibody (68250-1-Ig, Clone: 4A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman kidney tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68250-1-Ig targets Podocalyxin in IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Podocalyxin fusion protein Ag12859 相同性解析による交差性が予測される生物種
Full Name podocalyxin-like
Calculated molecular weight 526 aa, 55 kDa
Observed molecular weight60-70 kDa
GenBank accession numberBC093730
Gene symbol Podocalyxin
Gene ID (NCBI) 5420
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).

Protocols

Product Specific Protocols
IHC protocol for Podocalyxin antibody 68250-1-IgDownload protocol
IF protocol for Podocalyxin antibody 68250-1-IgDownload protocol
FC protocol for Podocalyxin antibody 68250-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols