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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    WB analysis of human brain using 22099-1-AP (same clone as 22099-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 22099-1-AP (Podoplanin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22099-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    WB analysis of human brain using 22099-1-AP (same clone as 22099-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 22099-1-AP (Podoplanin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22099-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    WB analysis of human placenta using 22099-1-AP (same clone as 22099-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 22099-1-AP (Podoplanin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IHC staining of human appendicitis using 22099-1-AP (same clone as 22099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IHC staining of human appendicitis using 22099-1-AP (same clone as 22099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IHC staining of human lymphoma using 22099-1-AP (same clone as 22099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IHC staining of human lymphoma using 22099-1-AP (same clone as 22099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22099-1-AP (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22099-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IF Staining of human placenta using 22099-1-AP (same clone as 22099-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Podoplanin antibody (22099-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22099-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using Podoplanin Polyclonal antibody (22099-1-AP)

    IF Staining of human placenta using 22099-1-AP (same clone as 22099-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Podoplanin antibody (22099-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22099-1-PBS in a different storage buffer formulation.

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Podoplanin Polyclonal antibody, PBS Only

Podoplanin Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 22099-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

PDPN, PSEC0003, PA2.26 antigen, Aggrus, 29kDa cytosolic podoplanin intracellular domain

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22099-1-PBS targets Podoplanin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Podoplanin fusion protein Ag17307 相同性解析による交差性が予測される生物種
Full Name podoplanin
Calculated molecular weight 238 aa, 25 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC022812
Gene Symbol Podoplanin
Gene ID (NCBI) 10630
RRIDAB_2878995
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86YL7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.