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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD155/PVR Monoclonal antibody (66913-1-Ig)

    WB analysis using 66913-1-Ig (same clone as 66913-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66913-1-Ig (CD155/PVR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66913-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using CD155/PVR Monoclonal antibody (66913-1-Ig)

    WB analysis of K-562 using 66913-1-Ig (same clone as 66913-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66913-1-Ig (CD155/PVR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66913-1-PBS in a different storage buffer formulation.

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CD155/PVR Monoclonal antibody, PBS Only

CD155/PVR Monoclonal Antibody for WB, ELISA
Cat No. 66913-1-PBS
Clone No.1F12A1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

CD155, FLJ25946, HVED, Necl 5, NECL5, Nectin like protein 5, poliovirus receptor, PVR, PVS, TAGE4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66913-1-PBS targets CD155/PVR in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD155/PVR fusion protein Ag28303 相同性解析による交差性が予測される生物種
Full Name poliovirus receptor
Calculated molecular weight 45 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC015542
Gene Symbol CD155/PVR
Gene ID (NCBI) 5817
RRIDAB_2882240
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15151
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.