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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Prealbumin/transthyretin Monoclonal antibody, PBS Only

Prealbumin/transthyretin Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H8C4

Cat no : 66108-1-PBS

Synonyms

ATTR, HsT2651, PALB, Prealbumin, Prealbumin/transthyretin, TBPA, transthyretin, TTR

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using Prealbumin/transthyretin Monoclonal antibody (66108-1-Ig)

    WB analysis of human brain using 66108-1-Ig (same clone as 66108-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Prealbumin/transthyretin Monoclonal antibody (66108-1-Ig)

    WB analysis of human heart using 66108-1-Ig (same clone as 66108-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using Prealbumin/transthyretin Monoclonal antibody (66108-1-Ig)

    WB analysis of fetal human brain using 66108-1-Ig (same clone as 66108-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Prealbumin/transthyretin Monoclonal antibody (66108-1-Ig)

    IHC staining of human liver using 66108-1-Ig (same clone as 66108-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Prealbumin/transthyretin Monoclonal antibody (66108-1-Ig)

    IHC staining of mouse liver using 66108-1-Ig (same clone as 66108-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Prealbumin/transthyretin Monoclonal antibody (66108-1-Ig)

    IHC staining of mouse liver using 66108-1-Ig (same clone as 66108-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Prealbumin/transthyretin Monoclonal antibody (66108-1-Ig)

    IHC staining of human liver using 66108-1-Ig (same clone as 66108-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-12 cells using Prealbumin/transthyretin Monoclonal antibody (66108-1-Ig)

    FC experiment of PC-12 using 66108-1-Ig (same clone as 66108-1-PBS)

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Prealbumin/transthyretin (66108-1-Ig, Clone:1H8C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66108-1-PBS targets Prealbumin/transthyretin in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Prealbumin/transthyretin fusion protein Ag19232 相同性解析による交差性が予測される生物種
Full Name transthyretin
Calculated molecular weight 147 aa, 16 kDa
Observed molecular weight 14-16 kDa, 28-33 kDa
GenBank accession numberBC020791
Gene symbol TTR
Gene ID (NCBI) 7276
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. TTR can be expressed as monomer (14-16 kDa), dimmer (28-33 kDa), or tetramer around 55-60 kDa. (PMID: 22103483)