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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Prestained Protein Marker (10-180 kDa)

Molecular Weight

10-180 kDa

Detection Method

Colorimetric, Fluorescence

Number of Markers

10

Stain Type

3 colors: Blue, Red, Green

Cat no : PL00001

Validation Data Gallery

  • The prestained protein ladder was resolved in a 10-20% Tris-glycine gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.

    The prestained protein ladder was resolved in a 10-20% Tris-glycine gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.

  • Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in observed molecular weight of the protein markers between different gel types.

    Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in observed molecular weight of the protein markers between different gel types.

  • The prestained protein ladders were resolved in an 8-16% Tris-glycine gel (SDS-PAGE). The image shows a comparison of the migration patterns on the gel for our standard (PL00001), broad (PL00002), and extra broad (PL00003) range ladders.

    The prestained protein ladders were resolved in an 8-16% Tris-glycine gel (SDS-PAGE). The image shows a comparison of the migration patterns on the gel for our standard (PL00001), broad (PL00002), and extra broad (PL00003) range ladders.

  • 2 uL protein ladders were loaded in the 8%-18% Tris-glycine gel, then electrophoresed and transformed into the PVDF membrane. After blocking, this dual-channel image was taken directly by the Bio-Rad ChemiDoc MP Imaging System in the 550 nm (for 75 kDa and 310 kDa) and 680 nm (except 75 kDa and 310 kDa) range.

    2 uL protein ladders were loaded in the 8%-18% Tris-glycine gel, then electrophoresed and transformed into the PVDF membrane. After blocking, this dual-channel image was taken directly by the Bio-Rad ChemiDoc MP Imaging System in the 550 nm (for 75 kDa and 310 kDa) and 680 nm (except 75 kDa and 310 kDa) range.

Product Information

The PL00001 Prestained Protein marker is a ready to use three-color protein standard with 10 prestained proteins covering a wide range of molecular weights from 10 to 180 kDa. The PL00001 Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, (PVDF, nylon, or nitrocellulose) and for estimating the size of proteins. The PL00001 Prestained Protein marker is also suitable for fluorescence WB detection.

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Storage

Store product at 4℃ for up to 12 weeks. For longer storage, aliquot and store at -20℃ for up to 1 year.

Usage

The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 uL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.

Detection Method

Colorimetric

Molecular Weight

~ 10, 15, 25, 35, 45, 60, 75, 100, 140, 180 kDa

Number of Markers

10

Size Range

10 to 180

Stain Type

3 colors: Blue, Red, Green

Cited in Article as

pl00001, Prestained Protein Marker (10-180 kDa), Proteintech, IL, USA

Documentation

SDS
Prestained Potein Marker 10-180kDa

Publications

ApplicationTitle

Cell Rep

A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression

Authors - Ling Zhang
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WB

Food Chem

Fabrication of grape seed proanthocyanidin-loaded W/O/W emulsion gels stabilized by polyglycerol polyricinoleate and whey protein isolate with konjac glucomannan: Structure, stability, and in vitro digestion

Authors - Hong Zhuang
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J Ethnopharmacol

Pharmacodynamics and pharmacological mechanism of Moluodan concentrated pill in the treatment of atrophic gastritis: A network pharmacological study and in vivo experiments

Authors - Ni Lou
View Article

Front Immunol

Roles of RAGE/ROCK1 Pathway in HMGB1-Induced Early Changes in Barrier Permeability of Human Pulmonary Microvascular Endothelial Cell.

Authors - Meng-Jiao Zhao
View Article

Sci Rep

Atypical chemokine receptor 2 expression is directly regulated by hypoxia inducible factor-1 alpha in cancer cells under hypoxia

Authors - Alice Benoit
View Article

J Ethnopharmacol

The mechanisms of action of WeiChang'An Pill (WCAP) treat diarrhoea-predominant irritable bowel syndrome (IBS-D) using network pharmacology approach and in vivo studies.

Authors - Yan Chen
View Article