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  • The prestained protein ladder was resolved in a 10-20% Tris-glycine gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.

    The prestained protein ladder was resolved in a 10-20% Tris-glycine gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.

  • Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in the observed molecular weight of the protein markers between different gel types, including Tris-Glycine gel, Tris-Acetate gel, and Bis-Tris gel.

    Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in the observed molecular weight of the protein markers between different gel types, including Tris-Glycine gel, Tris-Acetate gel, and Bis-Tris gel.

  • The prestained protein ladders were resolved in an 8-16% Tris-glycine gel (SDS-PAGE). The image shows a comparison of the migration patterns on the gel for our standard (PL00001), broad (PL00002), and extra broad (PL00003) range ladders.

    The prestained protein ladders were resolved in an 8-16% Tris-glycine gel (SDS-PAGE). The image shows a comparison of the migration patterns on the gel for our standard (PL00001), broad (PL00002), and extra broad (PL00003) range ladders.

  • 2 uL protein ladders were loaded in the 8%-18% Tris-glycine gel, then electrophoresed and transformed into the PVDF membrane. After blocking, this dual-channel image was taken directly by the Bio-Rad ChemiDoc MP Imaging System in the 550 nm (for 75 kDa and 310 kDa) and 680 nm (except 75 kDa and 310 kDa) range.

    2 uL protein ladders were loaded in the 8%-18% Tris-glycine gel, then electrophoresed and transformed into the PVDF membrane. After blocking, this dual-channel image was taken directly by the Bio-Rad ChemiDoc MP Imaging System in the 550 nm (for 75 kDa and 310 kDa) and 680 nm (except 75 kDa and 310 kDa) range.

Prestained Protein Marker (10-180 kDa)

Cat No. PL00001

Publications

43

Molecular Weight

10-180 kDa

Detection Method

Colorimetric, Fluorescence

Number of Markers

10

Stain Type

3 colors: Blue, Red, Green

Size: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Product Information

The PL00001 Prestained Protein marker is a ready to use three-color protein standard with 10 prestained proteins covering a wide range of molecular weights from 10 to 180 kDa. The PL00001 Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, (PVDF, nylon, or nitrocellulose) and for estimating the size of proteins. The PL00001 Prestained Protein marker is also suitable for fluorescence WB detection.

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Storage

Store product at 4℃ for up to 12 weeks. For longer storage, aliquot and store at -20℃   for up to 1 year.

Usage

The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 uL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.

Detection Method

Colorimetric

Molecular Weight

~ 10, 15, 25, 35, 45, 60, 75, 100, 140, 180 kDa

Number of Markers

10

Size Range

10 to 180

Stain Type

3 colors: Blue, Red, Green

Cited in Article as

pl00001, Prestained Protein Marker (10-180 kDa), Proteintech, IL, USA

Publications

ApplicationTitle

J Extracell Vesicles

Dendritic Cell Derived-Extracellular Vesicles Engineered to Express Interleukin-12 and Anti-CTLA-4 on Their Surface for Combinational Cancer Immunotherapy

Authors - Jiangbin Chen
View Article

J Adv Res

Discovery of novel selective PI3Kγ inhibitors through combining machine learning-based virtual screening with multiple protein structures and bio-evaluation

Authors - Jingyu Zhu
View Article

Adv Sci (Weinh)

Injectable biodegradable microcarriers for iPSC expansion and cardiomyocyte differentiation

Authors - Annalisa Bettini
View Article
WB

Acta Biomater

Targeting Delivery of Mifepristone to Endometrial Dysfunctional Macrophages for Endometriosis Therapy

Authors - Meng Zhang
View Article
WB

Food Chem

Fabrication of grape seed proanthocyanidin-loaded W/O/W emulsion gels stabilized by polyglycerol polyricinoleate and whey protein isolate with konjac glucomannan: Structure, stability, and in vitro digestion

Authors - Hong Zhuang
View Article
WB

Sci China Life Sci

CRISPR screening identifies protein methylation and ubiquitination modifications that modulate aflatoxin B1 cytotoxicity

Authors - Huaqiang Yang
View Article
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