Prestained Protein Marker (10-180 kDa)

A very good product and great support from the ProteinTech team. I'm sure this will be very useful for my next experiments during my thesis.

• Applications: Western Blot

I like the 3 colours of the marker because the resulting asymmetry makes keeping track of the gel orientation very easy, compared to symmetrical markers of other manufacturers.

• Applications: Western Blot

The ladder is great because the three colors help to easily distinguish the different molecular weights

• Applications: Western Blot

Excellent quality

• Applications: Western Blot

I think this product will be very useful to determine the size of proteins inside extracellular vesicles produced by my cells.

• Applications: Western Blot

THE PRE-STAINED PROTEIN MARKER THAT I RECEIVED WILL BE VERY USEFUL FOR MY PHD PROJECT.

• Applications: Cell culture

Très bon produit merci !

• Applications: Western Blot,Cell culture

Le produit est très bien, je pense en reprendre lorsque je l'aurai fini.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000

It is a really good marker, the transfert to the nitrocellulose is very clean

• Applications: Western Blot

After using the Proteintech PL00001 Prestained Protein Marker, I found the color-coded bands to be its standout feature. Each band is brightly colored and distinct, making it incredibly easy to track the gel’s progress in real time and identify molecular weights without additional staining. This color clarity not only saves time but also improves accuracy, especially when distinguishing between closely spaced bands. It's reliable and stable across multiple uses, making it an ideal choice for routine SDS-PAGE and western blot.

• Applications: Western Blot

One of my fav products. I normally use 3 ul in a well and it looks wonderful both in the gel and after transfer to the membrane. Easy to check the MW looking at the colours.

• Applications: Western Blot
• Cell Tissue Type: -

Excellent quality for the price

• Applications: Western Blot,Protein purification

Great ladder!

• Applications: Western Blot

Best ladder on the market - reliable results every time even with very sparing loading (2-3ul per protein gel), very competitive price. The packaging Proteintech products come in is excellent.

• Applications: Protein purification

protein ladder is perfectly visible, 2-3 ul is enough for one gel.

• Applications: Western Blot,Immunoprecipitation,Protein purification
• Cell Tissue Type: thylakoid membrane

The pre-stained protein marker yielded a nice result in my Western Blot assay.

• Applications: Western Blot

This protein ladder is great! The volume needed to get bright , clear bands transferred to PVDF is minimal compared to other ladders I have used. I will be transitioning to exclusively using this ladder on future blots.

• Applications: Western Blot

Excellent protein marker. Sharpness, quality and excellent value for money.

• Applications: Western Blot
• Cell Tissue Type: L929

The ladder works very good. Clear bands with bright colors. I recommend it.

• Applications: Western Blot

Fantastic ladder! Also with low amounts all the weights are clear!

• Applications: Western Blot

Works perfectly and no issues with transferring onto the PVDF membrane for Western Blot

• Applications: Western Blot

5µl of the ladder were resolved on 4-20% polyacrylamide gel. Asymmetric position of the bands and 3 different colours make it handful to use for proteins in the range 10-180 kDa.

• Applications: Western Blot

The Prestained Protein Marker works really good. You can see the 3 different colors in the acrilamide gel ( 12 %) and get straight bands. For protein between 10 up to 180 kDa it is a nice marker.

• Applications: Western Blot
• Cell Tissue Type: Hek cells

Good protein ladder and good size distribution

• Applications: Western Blot

The ladder works well and is easy to read due to triple colour banding pattern. Its very useful that the pattern is assymetric to allow for easy reading. The ladder also works for Fluorescent western blot developing. In that case you can clearly discern the banding pattern by the presence of thick bands. Personally I would however use the broad range ladder so that you can also clearly seperate larger proteins.

• Applications: Western Blot

The ladder properly worked in an acrilamide 10% gel. The pressence of three different colors facilitate the tracking of the molecular weight. I recommend this product.

• Applications: Western Blot

Great product

• Applications: Western Blot

Good product. Easy to use and the marker bands are well defined. Quick delivery from Proteintech

• Applications: Western Blot
• Cell Tissue Type: HNSCC cancer cells

Very good marker with clean and nice bands.

• Applications: Western Blot
• Cell Tissue Type: HEK293T cells

Very good protein ladder. Clear bands for our typical working range between 10 to 180 kDa

• Applications: Western Blot

Just receive the protein ladder and immediately tried with a WB! it work perfectly and it is clearly visible

• Applications: Western Blot

Very good coloured protein ladder. I loaded 1.5 ul on a SDS gel PAGE and transferred to nitrocellulose membrane and the ladder was extremely clean and visible. I will load less next time, 1 ul should be more than enough

• Applications: Western Blot

not equal as other used but ok

• Applications: Western Blot

Very versatile ladder! I do enjoy the quantity and positioning of the bands, especially under 50kDa. This ladder allowed me to assign MW to proteins which normally fall between the bands

• Applications: Western Blot
• Cell Tissue Type: C. elegans

Very good prestained protein marker. Clear bands and wide range.

• Applications: Western Blot

This prestained protein ladder works really good in 10-180kDa range

• Applications: Western Blot

The samples were run on a 15% SDS-PAGE

• Applications: Western Blot
• Cell Tissue Type: HeLa cells

It works very well!

• Applications: Western Blot

Accurate MW range, great staining

• Applications: Western Blot
• Cell Tissue Type: mouse embryonic stem cells

Great protein marker! Makes cutting membranes very easy.

• Applications: Western Blot
• Primary Antibody Dilution: N/A
• Cell Tissue Type: N/A

I found the product really promising. I compared it to PageRuler that we use traditionally and it matches the results quite perfectly.

• Applications: Other

Great protein ladder. Works exactly as expected :)

• Applications: Protein purification

I used it and it worked very well, I recommend it

• Applications: Western Blot

Reliable marker with good sharps bands and good signal even with low quantity

• Applications: Western Blot

It works very well giving sharp and reliable bands

• Applications: Western Blot

The marker works very good. It was easy to distinguish different bands. I used it to look at my protein of interest and house keeping, kDA bands looks very good.

• Applications: Western Blot
• Cell Tissue Type: Lymphocyte

Perfect! It does its job. I've used it for a western blot. I recommended it.

• Applications: Western Blot
• Cell Tissue Type: HeLa

Used for WB, worked perfectly fine, also compared with my previous protein marker. Totally recommend

• Applications: Western Blot

Great protein ladder. Sharp bands and bright colors after transfer to nitrocellulose membrane. It comes in ready-to-use format, which is quite convenient.

• Applications: Western Blot

very clear ladder, easy to see on PVDF membrane. Worth the price.

• Applications: Western Blot

The ladder works very well. I’ve used it to look at p53 isoforms. Good colors.

• Applications: Western Blot

The protein ladder worked well and the 3 different colors made the detection easier.

• Applications: Western Blot

The protein ladder is well made and mantains color for long time

• Applications: Western Blot

i tried this as part of the phd free trial kit and i'm very satisfied of the result, i'm considering ordering this again

• Applications: Western Blot

Clear, bright bands. Nicely colour coded for ease of use.

• Applications: Western Blot

Prestained proteine marker works very well in a Wb gel (1mm) with a persistent color also After washing

• Applications: Western Blot
• Primary Antibody Dilution: 4 uL for Gel 1 mm

excellent protein markers

• Applications: Western Blot

The marker works really well, it is precise and bright. Excellent product. Recommended.

• Applications: Western Blot

Great Ladder very clean and precise, I used 5uL and the color on an homemade 12% gel and the resolution it has been perfect

• Applications: Western Blot

Very defined bands

• Applications: Western Blot

Markers ran well and the color scale was clearly visible

• Applications: Western Blot

Markers worked very well, with bands well defined

• Applications: Western Blot

Ladder separated out well, colours made it really easy to read. I really like this ladder!

• Applications: Other

First time trial. Positive impression. The ladder is just comparable to the one we normally use (other brand). It was suggested to load 3-5uL but personally I would use 10uL for a more definite ladder. No complaints.

• Applications: Western Blot
• Cell Tissue Type: Total protein extracts from S. cerevisiae cells

Worked great for Western Blot!

• Applications: Western Blot

The prestained protein marker is well visibile and the color brightness is conserved in time

• Applications: Western Blot
• Cell Tissue Type: Melanoma

prestained protein marker maintains colour brightness even after strippimng procedures.

• Applications: Western Blot
• Cell Tissue Type: Dendritic cells

good ladder, transfers well

• Applications: Western Blot

Worked out great, nice band separation

• Applications: Other

Ladder ran really nicely and gave clear and well defined bands on my gel

• Applications: Western Blot

Worked well.

• Applications: Western Blot

The prestained protein ladder shows clear bands for a reasonable range of molecular weights. Including bands for smaller proteins might be useful for some cases.

• Applications: Western Blot,Other

Protein ladder used for SDS PAGE 10%, minigel, 1mm thikness. 10ul of ladder were loaded after running the gel the bands higher then the 70 kDA band disappeared

• Applications: Western Blot

Very nice ladder. I tried using only 2uL and the bands were still clear.

• Applications: Western Blot

Cell whole lysate labelled with No-Stain (20 ug protein), VWR VI marker 9 uL, Proteintech PL00001 5 uL.

• Applications: Western Blot

Perfect marker for protein gels, crispy bands

• Applications: Western Blot

Very good ladder. The bands are well separated and visible.

• Applications: Western Blot

The bands are nicely separated and colorful.I used 5ul but looks like 2ul would be sufficient to see the bands.

• Applications: Western Blot

I requested a sample of Prestained protein marker 10-180 kDa) and received it in less than a week. The product is amazing, after loading only 3 uL (as I usually do), SDS-PAGE and transfer to membrane resulted in sharp and bright bands. I highly recommend this product.

• Applications: Western Blot

Excellent Prestained Protein Marker

• Applications: Western Blot
• Cell Tissue Type: MCEC

Works exactly as you'd expect it to, no complaints. Colours are vibrant and clear. Used in SDS-PAGE. This is a higher concentration than I'm used to (2-5x more concentrated at least), you only need to use 1-3 µL in a 50µL well

• Applications: Other
• Cell Tissue Type: 12% 10 well 50 µL precast protein gel

Great prestained protein ladder with clear colourful bands. Good separation in SDS-PAGE and nice transfer.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Liver

Really clear protein ladder that ran well, colours help visualisation on SDS-PAGE gel

• Applications: Other
• Cell Tissue Type: LOBSTR BL21 E.Coli

Nice ladder, good seperation

• Applications: Western Blot

The protein ladder worked comparably well to the ladder that we currently use from BioRad. Unfortunately, the BioRad ladder is cheaper to buy in bulk but I wouldn't hesitate to use this one.

• Applications: Western Blot
• Cell Tissue Type: N/A

in lane on the right, tested against precision plus protein dual color standard from Bio-Rad, cat# 1610374 (left). red and green channel fluorescence in black and white included, ladder looked much better in the red channel.

• Applications: Western Blot

Fantastic Protein Marker. Highly recommendable for any use: gels, blotting, etc. etc. etc. Clear colourful bands.

• Applications: Western Blot

Great protein marker, easy to use. It does the job!

• Applications: Western Blot

The marker is easy to use and the bands are nicely separated.

• Applications: Western Blot

Product reacted well to the experiment and yielded good results.

• Applications: Other
• Cell Tissue Type: Cnacerous

Ladder separates and aligns great my known protein.

• Applications: Western Blot
• Cell Tissue Type: GBM cells

I compared with other company ladder and looks pretty good, with only 4uL. Photo form a 4-20% TGX gel

• Applications: Western Blot

this product works great.

• Applications: Western Blot

I like this new ladder. Just have some difficulties to observe the green ladder during migration but become better after transfer.

• Applications: Western Blot
• Cell Tissue Type: NRVM

It is great.. worked well on SDS-PAGE

• Applications: Western Blot

very good working

• Applications: Western Blot

3ul of ladder loaded onto 12% acrlyamide gel. Transfer to nitrocellulose membrane and stained with ponceau to assess total protein, as seen in image.Ladder was clearly visible in both the gel and the membrane.

• Applications: Western Blot

Works well both ín SDS-polyacrylamide gel and on PVDF membranes. Load 1 ul and still clearly visible.

• Applications: Western Blot

Loaded 5uL of protein ladder into left lane on 12.5% gel, followed by transfer to nitrocellulose membrane.Ladder was clearly visible in both gel and membrane. Image captured after ponceau stain.

• Applications: Western Blot

I used this marker with Biorad precasted gels 4-15% and it separates perfectly allowing you to compare the size of your protein in a pretty accurate way. I would definitely order it for next experiments.

• Applications: Western Blot
• Cell Tissue Type: Protein extract

I have used this ladder a lot and it always worked reliably and consitent and I never had any issues. The bands show up strong and clearly as expected

• Applications: Other
• Cell Tissue Type: Primary Proximal Tubule cell lysates

It is great.

• Applications: Western Blot

This protein ladder was working great, it has a single reference band which avoid confusions of comparing between two or three reference bands. And it runs the same way as shown in datasheet if we run the marker in different percentages of gels.

• Applications: Western Blot

I wanted to test out 2,2,2-trichloroethanol in my resolving gel and it was good so I asked if the student would like to continue on with procedure and transfer to blot they did not have time so we did not go further than the gel electrophoresis.The ladder was bright and you could see the three distinct colors of the various bands, blue, green and red. I would definitely continue to use this product in my western blot analyses

• Applications: Western Blot
• Cell Tissue Type: Dorsal Root Ganglia Cells

Protein Ladder works very well. I have used 4 - 5ul and both band clearly visible.With different colors easy to measure the weight of sample protein.

• Applications: Other
• Cell Tissue Type: Insect midgut

Good staining and good bands quality

• Applications: Western Blot

The ladder worked perfectly. There were no differences in performance between this ladder and previous ladder I have used. Coloured markers were nice for images.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse hippocampus

The ladder runs well in SDS gel loading 2,5 ul, the bands match well with the tested antibodies (actin, sigma1r, VDAC, IP3R, catalase)

• Applications: Western Blot
• Cell Tissue Type: Neural stem progenitor cells

I used the protein ladder and it works fine for me

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U87-MG

This multicolored protein ladder was easy to see with only 5 uL loaded into the well.

• Applications: Other
• Cell Tissue Type: Urine

Work well on SDS-PAGE

• Applications: Western Blot

Good!

• Applications: Western Blot

I used 5ul, the bands are clearly visible and the color bands are helpful to detect the molecular weight easily

• Applications: Western Blot

Gives clear bands on polyacrylamide gel, and on nitrocellulose membrane after standard 1h wet transfer.

• Applications: Western Blot

The ladder ran as expected and was easy to detect on the gel.

• Applications: Western Blot
• Cell Tissue Type: Mouse tissue

Prestained protein marker can be used 4 ul per lane. I saw bright, robust bands on my PVDF that lasted through 3 rounds of antibody incubations and ECL.

• Applications: Western Blot
• Cell Tissue Type: n/a

1 lane : predestined protein marker from proteintech (5ul)2 lane : protein marker from lab (10ul)3, 4 lane : 5ug, 10ug Postmortem brain tissue

• Applications: Western Blot
• Primary Antibody Dilution: predestined protein marker
• Cell Tissue Type: Postmortem brain tissue

It is easily visible and the color-coded bands are really helpful.

• Applications: Western Blot

The markers look nice and transferred well for Western blot.

• Applications: Western Blot

I needed a ladder with a 75 kDa band. The proteintech ladder worked very well for my purpose.

• Applications: Other

The Ladder resolves very well on 12% SDS-PAGE gel. It transfers very efficiently onto the PVDF membrane upon Western transfer. The ladder is retained on the blot even after stripping the blot. However, upon stripping the green band of the ladder disappears.

• Applications: Western Blot

I recently used the protein ladder and am happy to say it works as same as product from Bio Rad dual color standards.

• Applications: Western Blot
• Cell Tissue Type: mouse hupothalamic N39 cells

Works great

• Applications: Western Blot

I tested this product in a wb gel at 10%, the bands were clearly visible and clear

• Applications: Western Blot
• Cell Tissue Type: erythroid cells

The ladder worked beautifully in our hands. The range of weights is very suitable for our studies to try fing new targets for anti-epileptic drugs

• Applications: Western Blot
• Cell Tissue Type: Brain tissue

I have used this as a replacement for PageRuler Plus for a western with a large (~200kDa) protein, and it worked brilliantly.

• Applications: Western Blot

Worked perfectly well and will definitely consider ordering more

• Applications: Western Blot

Separetion of the bands was nice, th green color of the ~23kDa band was a bit less visible the other 2 colours but it was ok.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000

I've used the Pritein Ladder in several Western Blot analysis and I can now say, that the bands are very clearly visible on gel and on the membrane, what makes the protein identification faster and cutting membranes easier.

• Applications: Western Blot,

5 micro L of ladder loaded on protein 4-15% tris glycine gel . Gel wet transferred onto 0.22um nitrocellulose membrane . Membrane was stained with amido black and destained with 20% methanol 10% acetic acid Protein marker is still visible with c colours bands as well still good product

• Applications: Western Blot,
• Primary Antibody Dilution: 5mico L loaded
• Cell Tissue Type: NA

The ladder ran and separated well on the gel. I compared it to my normal protein ladder from ThermoFisher.I didn't image it in the end, since my actual samples didn't work that time!

• Applications: Western Blot,

Molecular weight ladder worked well overall, when we loaded 5ul of the pre-stained ladder. However, the high molecular bands (140-180kDa) were very faint, and then completely disappeared after the PVDF membranes were stripped to re-probe for a second marker.

• Applications: Western Blot,
• Cell Tissue Type: Murine brain homogenate

it works well for Western blot

• Applications: Western Blot,

Very useful. I used only 3 ul and yet it looked great on the gel. I was able to follow the run and separate well the molecular weights of my interest. Having different colors makes it very easy to read.

• Applications: Western Blot,

The ladder works well, detecting the bands at the expected size

• Applications: Western Blot,
• Cell Tissue Type: Lymphoma cell lines

Nice ladder! Very easy to see, both in the gel (while running and before transfer) and in the membrane (after transfer). The transfer from the gel to membrane was almost 100%.

• Applications: Western Blot,
• Primary Antibody Dilution: 3ul per lane

A good protein ladder

• Applications: Western Blot,
• Cell Tissue Type: retina

Good ladder run on SDS-PAGE gel. Thanks!

• Applications: Other,

I liked this product compared to another one I was using before because of the 3 colors it uses.

• Applications: Western Blot,

Excellent ladder, I liked it better than the one we currently use (SeeBlue Plus2 ladder) as the larger bands are more easily visible.

• Applications: Western Blot,

Ladder worked really well, very clear both on the gel and the membrane after transfer.

• Applications: Western Blot,

Great protein ladderClear running and the three different colours make it easy to distinguish different weights for antibody probing/ cutting membrane

• Applications: Western Blot,
• Cell Tissue Type: Human

Great ladder, clear and useful. Small amount needed.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:20000