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Progranulin/PGRN Polyclonal antibody, PBS Only

Name: Progranulin/PGRN Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 18410-1-PBS

Progranulin/PGRN Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Review Product

Cat no : 18410-1-PBS

Synonyms

Validation Data Gallery

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AllWBIHC

WB analysis using 18410-1-AP (same clone as 18410-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 18410-1-AP (same clone as 18410-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
WB analysis of mouse lung using 18410-1-AP (same clone as 18410-1-PBS)
mouse lung tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
WB analysis of U-937 using 18410-1-AP (same clone as 18410-1-PBS)
U-937 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 18410-1-AP (same clone as 18410-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 18410-1-AP (same clone as 18410-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 18410-1-AP (same clone as 18410-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
WB analysis of THP-1 using 18410-1-AP (same clone as 18410-1-PBS)
THP-1 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 18410-1-AP (same clone as 18410-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 18410-1-AP (same clone as 18410-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
WB analysis of human saliva using 18410-1-AP (same clone as 18410-1-PBS)
human saliva were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 18410-1-AP (same clone as 18410-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 18410-1-AP (same clone as 18410-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 18410-1-AP (same clone as 18410-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 18410-1-AP (same clone as 18410-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 18410-1-AP (same clone as 18410-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 18410-1-AP (same clone as 18410-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 18410-1-AP (same clone as 18410-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 18410-1-AP (same clone as 18410-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 18410-1-AP (same clone as 18410-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 18410-1-AP (same clone as 18410-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18410-1-PBS in a different storage buffer formulation.

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Cat No. 18410-1-PBS

Size/Concentration

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Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18410-1-PBS targets Progranulin/PGRN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Progranulin/PGRN fusion protein Ag13178 相同性解析による交差性が予測される生物種
Full Name	granulin
Calculated molecular weight	64 kDa
Observed molecular weight	60-70 kDa
GenBank accession number	BC000324
Gene symbol	Granulin
Gene ID (NCBI)	2896
RRID	AB_10598161
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Progranulin/PGRN Polyclonal antibody, PBS Only

Progranulin/PGRN Polyclonal Antibody for WB, IHC, Indirect ELISA

Validation Data Gallery

WB analysis using 18410-1-AP (same clone as 18410-1-PBS)

WB analysis of mouse heart using 18410-1-AP (same clone as 18410-1-PBS)

WB analysis of mouse lung using 18410-1-AP (same clone as 18410-1-PBS)

WB analysis of U-937 using 18410-1-AP (same clone as 18410-1-PBS)

WB analysis of HEK-293 using 18410-1-AP (same clone as 18410-1-PBS)

WB analysis of PC-3 using 18410-1-AP (same clone as 18410-1-PBS)

WB analysis of rat brain using 18410-1-AP (same clone as 18410-1-PBS)

WB analysis of THP-1 using 18410-1-AP (same clone as 18410-1-PBS)

WB analysis of A431 using 18410-1-AP (same clone as 18410-1-PBS)

WB analysis of HeLa using 18410-1-AP (same clone as 18410-1-PBS)

WB analysis of human saliva using 18410-1-AP (same clone as 18410-1-PBS)

IHC staining of human pancreas cancer using 18410-1-AP (same clone as 18410-1-PBS)

IHC staining of human pancreas cancer using 18410-1-AP (same clone as 18410-1-PBS)

IHC staining of human kidney using 18410-1-AP (same clone as 18410-1-PBS)