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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Prohibitin 2 Monoclonal antibody (66424-1-Ig)

    WB analysis using 66424-1-Ig (same clone as 66424-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Prohibitin 2 Monoclonal antibody (66424-1-Ig)

    WB analysis of HeLa using 66424-1-Ig (same clone as 66424-1-PBS)

    WB result of Prohibitin 2 antibody (66424-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Prohibitin 2 transfected HeLa cells. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Prohibitin 2 Monoclonal antibody (66424-1-Ig)

    WB analysis using 66424-1-Ig (same clone as 66424-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Prohibitin 2 Monoclonal antibody (66424-1-Ig)

    IHC staining of human breast cancer using 66424-1-Ig (same clone as 66424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Prohibitin 2 Monoclonal antibody (66424-1-Ig)

    IHC staining of human breast cancer using 66424-1-Ig (same clone as 66424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Prohibitin 2 Monoclonal antibody (66424-1-Ig)

    IHC staining of human heart using 66424-1-Ig (same clone as 66424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Prohibitin 2 Monoclonal antibody (66424-1-Ig)

    IHC staining of human heart using 66424-1-Ig (same clone as 66424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Prohibitin 2 Monoclonal antibody (66424-1-Ig)

    IF Staining of HepG2 using 66424-1-Ig (same clone as 66424-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin was labelled in red with Phalloidin. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Prohibitin 2 Monoclonal antibody (66424-1-Ig)

    IF Staining of HeLa using 66424-1-Ig (same clone as 66424-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Prohibitin 2 antibody (66424-1-Ig, Clone: 1D9C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Prohibitin 2 Monoclonal antibody, PBS Only

Prohibitin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66424-1-PBS
Clone No.1D9C7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PHB2, 1D9C7, BAP, B-cell receptor-associated protein BAP37, D prohibitin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66424-1-PBS targets Prohibitin 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Prohibitin 2 fusion protein Ag2977 相同性解析による交差性が予測される生物種
Full Name prohibitin 2
Calculated molecular weight 299 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC014766
Gene Symbol Prohibitin 2
Gene ID (NCBI) 11331
RRIDAB_2811041
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99623
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)