Prohibitin 2 Monoclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

WB result of Prohibitin 2 antibody (66424-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Prohibitin 2 transfected HeLa cells. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). F-actin was labelled in red with Phalloidin. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.