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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Prohibitin Monoclonal antibody, PBS Only

Prohibitin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D3G5

Cat no : 60092-1-PBS

Synonyms

Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, PHB, PHB1, prohibitin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Prohibitin Monoclonal antibody (60092-1-Ig)

    WB analysis using 60092-1-Ig (same clone as 60092-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60092-1-Ig (Prohibitin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Prohibitin Monoclonal antibody (60092-1-Ig)

    WB analysis of HeLa using 60092-1-Ig (same clone as 60092-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    WB analysis of human placenta using 60092-1-Ig (same clone as 60092-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Prohibitin Monoclonal antibody (60092-1-Ig)

    WB analysis of HeLa using 60092-1-Ig (same clone as 60092-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human liver using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human liver using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60092-1-Ig (Prohibitin Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60092-1-Ig (Prohibitin Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human kidney using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human kidney using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60092-1-Ig(PHB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Prohibitin Monoclonal antibody (60092-1-Ig)

    IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60092-1-Ig(PHB antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Prohibitin Monoclonal antibody (60092-1-Ig)

    IF Staining of HeLa using 60092-1-Ig (same clone as 60092-1-PBS)

    Immunofluorescent analysis of Hela cells, using PHB antibody 60092-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60092-1-PBS targets Prohibitin in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Prohibitin fusion protein Ag1240 相同性解析による交差性が予測される生物種
Full Name prohibitin
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC013401
Gene symbol PHB
Gene ID (NCBI) 5245
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).