Pseudouridine Monoclonal antibody
Pseudouridine Monoclonal Antibody for ELISA, Dot Blot
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
ELISA, Dot Blot
Conjugate
Unconjugated
CloneNo.
1G7C7
Cat no : 68578-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive ELISA detected in | Pseudouridine |
Positive Dot Blot detected in | RNA |
Recommended dilution
Application | Dilution |
---|---|
Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:2000-1:20000 |
DOT BLOT | DOT BLOT : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68578-1-Ig targets Pseudouridine in ELISA, Dot Blot applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PTG 相同性解析による交差性が予測される生物種 |
Full Name | Pseudouridine |
Gene symbol | |
Gene ID (NCBI) | |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Pseudouridine is an isomer of the nucleoside uridine in which the uracil is attached via a carbon-carbon instead of a nitrogen-carbon glycosidic bond. Pseudouridine is the most abundant RNA modification in cellular RNA (PMID: 29104216). After transcription and following synthesis, RNA can be modified with over 100 chemically distinct modifications. These can potentially regulate RNA expression post-transcriptionally, in addition to the four standard nucleotides and play a variety of roles in the cell including translation, localization and stabilization of RNA. Pseudouridine in rRNA and tRNA has been shown to fine-tune and stabilize the regional structure and help maintain their functions in mRNA decoding, ribosome assembly, processing and translation (PMID: 10902565, PMID: 23391857, PMID: 28045575). Pseudouridine in snRNA has been shown to enhance spliceosomal RNA-pre-mRNA interaction to facilitate splicing regulation (PMID 27268497).