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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using QPCT Polyclonal antibody (18571-1-AP)

    WB analysis using 18571-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18571-1-AP (QPCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using QPCT Polyclonal antibody (18571-1-AP)

    WB analysis using 18571-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18571-1-AP (QPCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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QPCT Polyclonal antibody

QPCT Polyclonal Antibody for WB, ELISA
Cat No. 18571-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC, GCT, Glutaminyl cyclase, Glutamyl cyclase, QC, QPCT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inCaki-2 cells, human urine sample

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18571-1-AP targets QPCT in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen QPCT fusion protein Ag13416 相同性解析による交差性が予測される生物種
Full Name glutaminyl-peptide cyclotransferase
Calculated molecular weight 361 aa, 41 kDa
Observed molecular weight41 kDa, 35 kDa
GenBank accession numberBC047756
Gene Symbol QPCT
Gene ID (NCBI) 25797
RRIDAB_3669301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16769
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutaminyl-peptide cyclotransferase (QPCT) gene encodes glutaminyl cyclase (QC), an enzyme that plays a role in post-translational modification of proteins by converting N-terminal glutamate to pyroglutamine. QPCT plays a specific role in promoting tumor cell proliferation, migration and angiogenesis as well as in inhibiting tumor cell apoptosis in cancers such as thyroid cancer and renal cell carcinoma (PMID: 38417050). Human QPCT has two isoforms produced by alternative splicing, 41 kDa and 35 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for QPCT antibody 18571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols