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KD/KO Validated

QSOX1/Quiescin Q6 Polyclonal antibody

Name: QSOX1/Quiescin Q6 Polyclonal antibody
Brand: Proteintech
SKU: 12713-1-AP

QSOX1/Quiescin Q6 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12713-1-AP

Synonyms

QSOX1, UNQ2520/PRO6013, QSCN6, Q6, hQSOX

Validation Data Gallery

Filter:

AllWBIPIHCIF-P

WB analysis using 12713-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12713-1-AP (QSOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 12713-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12713-1-AP (QSOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 12713-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 12713-1-AP (QSOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of SW 1990 using 12713-1-AP
IP result of anti-QSOX1 (IP:12713-1-AP, 4ug; Detection:12713-1-AP 1:300) with SW 1990 cells lysate 2400ug.
IHC staining of human liver cancer using 12713-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12713-1-AP (QSOX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12713-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12713-1-AP (QSOX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse colon using 12713-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using QSOX1 antibody (12713-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse colon using 12713-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using QSOX1 antibody (12713-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 12713-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	SW 1990 cells, mouse heart tissue, human placenta tissue, rat heart tissue
Positive IP detected in	SW 1990 cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse colon tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 14 publications below
IHC	See 10 publications below
IF	See 3 publications below
CoIP	See 1 publications below

Product Information

12713-1-AP targets QSOX1/Quiescin Q6 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	QSOX1/Quiescin Q6 fusion protein Ag3396 相同性解析による交差性が予測される生物種
Full Name	quiescin Q6 sulfhydryl oxidase 1
Calculated molecular weight	747 aa, 83 kDa
Observed molecular weight	66-83kDa
GenBank accession number	BC017692
Gene symbol	QSOX1
Gene ID (NCBI)	5768
RRID	AB_2173314
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

QSOX1(Sulfhydryl oxidase 1) catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide.It has two isoforms with the molecular weight of 67 kda and 83 kDa,respectively. It is detected a band of 95 Kda in HT1080 cell lines (PMID:17331072).Isoform 1 is expressed on golgi apparatus membrane and isoform 2 is secreted (PMID:10708601). The isoform1(QSOX1a) is the full length of QSOX1, approximately 78 kd; and the isoform2(QSOX1b) is the splicing form, approximately 62kd(PMID:14633699; 2496424). QSOX1 is highly expressed in tumor cell lines but is not expressed in adjacent normal cells (PMID:21989104).

Protocols

Product Specific Protocols
WB protocol for QSOX1/Quiescin Q6 antibody 12713-1-AP	Download protocol
IHC protocol for QSOX1/Quiescin Q6 antibody 12713-1-AP	Download protocol
IF protocol for QSOX1/Quiescin Q6 antibody 12713-1-AP	Download protocol
IP protocol for QSOX1/Quiescin Q6 antibody 12713-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Redox Biol Quiescin sulfhydryl oxidase 1 promotes sorafenib-induced ferroptosis in hepatocellular carcinoma by driving EGFR endosomal trafficking and inhibiting NRF2 activation. Authors - Jialei Sun View Article
	WB	Antioxid Redox Signal The Emerging Role of QSOX1 in Cancer. Authors - Douglas F Lake View Article
human	IHC	Breast Cancer Res High expression of QSOX1 reduces tumorogenesis, and is associated with a better outcome for breast cancer patients. Authors - Pernodet Nicolas N View Article
human	WB,IHC	Breast Cancer Res Expression of quiescin sulfhydryl oxidase 1 is associated with a highly invasive phenotype and correlates with a poor prognosis in Luminal B breast cancer. Authors - Katchman Benjamin A BA View Article
human	IHC	Mod Pathol QSOX1 expression is associated with aggressive tumor features and reduced survival in breast carcinomas. Authors - Gøril Knutsvik View Article
human	IHC	Oncotarget Reduced QSOX1 enhances radioresistance in nasopharyngeal carcinoma. Authors - Lei Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

QSOX1/Quiescin Q6 Polyclonal antibody

QSOX1/Quiescin Q6 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Validation Data Gallery

WB analysis using 12713-1-AP

WB analysis using 12713-1-AP

WB analysis of human placenta using 12713-1-AP

IP experiment of SW 1990 using 12713-1-AP

IHC staining of human liver cancer using 12713-1-AP

IHC staining of human liver cancer using 12713-1-AP

IF Staining of mouse colon using 12713-1-AP

IF Staining of mouse colon using 12713-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Quiescin sulfhydryl oxidase 1 promotes sorafenib-induced ferroptosis in hepatocellular carcinoma by driving EGFR endosomal trafficking and inhibiting NRF2 activation.

The Emerging Role of QSOX1 in Cancer.

High expression of QSOX1 reduces tumorogenesis, and is associated with a better outcome for breast cancer patients.

Expression of quiescin sulfhydryl oxidase 1 is associated with a highly invasive phenotype and correlates with a poor prognosis in Luminal B breast cancer.

QSOX1 expression is associated with aggressive tumor features and reduced survival in breast carcinomas.

Reduced QSOX1 enhances radioresistance in nasopharyngeal carcinoma.

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