RAB11A Monoclonal antibody
RAB11A Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig, rabbit
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
4A4C9
Cat no : 67902-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, A431 cells, A549 cells, HeLa cells, pig brain tissue, rat brain tissue, rat cerebellum tissue, HepG2 cells, K-562 cells, rabbit brain tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67902-1-Ig targets RAB11A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RAB11A fusion protein Ag8879 相同性解析による交差性が予測される生物種 |
| Full Name | RAB11A, member RAS oncogene family |
| Calculated molecular weight | 216 aa, 24 kDa |
| Observed molecular weight | 24 kDa |
| GenBank accession number | BC013348 |
| Gene symbol | RAB11A |
| Gene ID (NCBI) | 8766 |
| RRID | AB_2918658 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB11A antibody 67902-1-Ig | Download protocol |
| IF protocol for RAB11A antibody 67902-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH X (Verified Customer) (10-17-2024) | A very good antibody to detect endo Rab11A from several sources.
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