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KD/KO Validated

RAB11A Polyclonal antibody

Name: RAB11A Polyclonal antibody
Brand: Proteintech
SKU: 20229-1-AP

RAB11A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20229-1-AP

Synonyms

RAB11A-Specific, RAB11, Rab 11, RAB11A/B, Rab-11

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 20229-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20229-1-AP
WB result of RAB11A-Specific antibody (20229-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB11A-Specific transfected HeLa cells.
WB analysis of rat brain using 20229-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-12 using 20229-1-AP
PC-12 cells were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 20229-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 20229-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 20229-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of SH-SY5Y using 20229-1-AP
IP result of anti-RAB11A-Specific (IP:20229-1-AP, 3ug; Detection:20229-1-AP 1:700) with SH-SY5Y cells lysate 3000ug.
IHC staining of human small intestine using 20229-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human small intestine using 20229-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 40x lens).
IHC staining of mouse brain using 20229-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 20229-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human small intestine using 20229-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human small intestine using 20229-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20229-1-AP (RAB11A-Specific antibody) at dilution of 1:200 (under 10x lens).
IF Staining of HEK-293 using 20229-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RAB11A-Specific antibody (20229-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Cat No. 20229-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293T cells, human brain tissue, mouse colon tissue, SH-SY5Y cells, rat brain tissue, PC-12 cells, HeLa cells, MCF-7 cells, mouse lung tissue
Positive IP detected in	SH-SY5Y cells
Positive IHC detected in	human small intestine tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 12 publications below
IHC	See 1 publications below
IF	See 13 publications below

Product Information

20229-1-AP targets RAB11A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	RAB11A, member RAS oncogene family
Calculated molecular weight	24 kDa
Observed molecular weight	24 kDa
GenBank accession number	NM_004663
Gene symbol	RAB11A
Gene ID (NCBI)	8766
RRID	AB_10666202
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939). The antibody is specific to RAB11A, and has no cross reaction to RAB11B.

Protocols

Product Specific Protocols
WB protocol for RAB11A antibody 20229-1-AP	Download protocol
IHC protocol for RAB11A antibody 20229-1-AP	Download protocol
IF protocol for RAB11A antibody 20229-1-AP	Download protocol
IP protocol for RAB11A antibody 20229-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	J Thromb Haemost Increased potency of recombinant VWF D'D3 albumin fusion proteins engineered for enhanced affinity for coagulation factor VIII. Authors - Jenny Chia View Article
human	WB	Proc Natl Acad Sci U S A ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting. Authors - Humbert Melissa C MC KD Validated View Article
		J Cell Biol BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia. Authors - William J Monis View Article
mouse	IF	EMBO Rep Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance. Authors - Ye Chen View Article
mouse	WB	Cell Mol Gastroenterol Hepatol Rab9 Mediates Pancreatic Autophagy Switch From Canonical to Noncanonical, Aggravating Experimental Pancreatitis. Authors - Olga A Mareninova View Article
human	IF	Br J Pharmacol An orally available hypoglycaemic peptide taken up by caveolae transcytosis displays improved hypoglycaemic effects and body weight control in db/db mice. Authors - Weisheng Lu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB11A Polyclonal antibody

RAB11A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 20229-1-AP

WB analysis of HeLa using 20229-1-AP

WB analysis of rat brain using 20229-1-AP

WB analysis of PC-12 using 20229-1-AP

WB analysis of human brain using 20229-1-AP

WB analysis of mouse colon using 20229-1-AP

WB analysis of SH-SY5Y using 20229-1-AP

IP experiment of SH-SY5Y using 20229-1-AP

IHC staining of human small intestine using 20229-1-AP

IHC staining of human small intestine using 20229-1-AP

IHC staining of mouse brain using 20229-1-AP

IHC staining of mouse brain using 20229-1-AP

IHC staining of human small intestine using 20229-1-AP

IHC staining of human small intestine using 20229-1-AP

IF Staining of HEK-293 using 20229-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Increased potency of recombinant VWF D'D3 albumin fusion proteins engineered for enhanced affinity for coagulation factor VIII.

ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia.

Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.

Rab9 Mediates Pancreatic Autophagy Switch From Canonical to Noncanonical, Aggravating Experimental Pancreatitis.

An orally available hypoglycaemic peptide taken up by caveolae transcytosis displays improved hypoglycaemic effects and body weight control in db/db mice.