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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAB11B Polyclonal antibody

RAB11B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28498-1-AP

Synonyms

Ras-related protein Rab-11B, Ras related protein Rab 11B, H YPT3, GTP-binding protein YPT3, GTP binding protein YPT3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB11B Polyclonal antibody (28498-1-AP)

    WB analysis using 28498-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28498-1-AP (RAB11B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAB11B Polyclonal antibody (28498-1-AP)

    WB analysis using 28498-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28498-1-AP (RAB11B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RAB11B Polyclonal antibody (28498-1-AP)

    IHC staining of human stomach using 28498-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28498-1-AP (RAB11B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RAB11B Polyclonal antibody (28498-1-AP)

    IF Staining of A549 using 28498-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RAB11B antibody (28498-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, PC-12 cells, SH-SY5Y cells, RAW 264.7 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, NIH3T3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28498-1-AP targets RAB11B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB11B fusion protein Ag25429 相同性解析による交差性が予測される生物種
Full Name RAB11B, member RAS oncogene family
Calculated molecular weight 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC110081
Gene symbol RAB11B
Gene ID (NCBI) 9230
RRIDAB_2881156
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAB11B antibody 28498-1-APDownload protocol
IHC protocol for RAB11B antibody 28498-1-APDownload protocol
IF protocol for RAB11B antibody 28498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Pathol Res Pract

SREBP2/Rab11s/GLUT1/6 network regulates proliferation and migration of glioblastoma

Authors - Chuandong Cheng
View Article