Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 19742-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 19742-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 19742-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of PC-12 using 19742-1-AP
PC-12 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 19742-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 19742-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 19742-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 19742-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 19742-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of PC-12 using 19742-1-AP
PC-12 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 19742-1-AP
IP result of anti-RAB11B-Specific (IP:19742-1-AP, 4ug; Detection:19742-1-AP 1:1000) with mouse brain tissue lysate 3200 ug.
IHC staining of human lung using 19742-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 19742-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 19742-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 19742-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 19742-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 19742-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 40x lens).

RAB11B-Specific Polyclonal antibody

RAB11B-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 19742-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

GTP binding protein YPT3, H YPT3, RAB11B, RAB11B-Specific, Ras related protein Rab 11B, YPT3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human brain tissue, HEK-293 cells, C6 cells, PC-12 cells, fetal human brain tissue, human testis tissue, mouse testis tissue, HeLa cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human lung tissue, human brain tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:300-1:600
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

19742-1-AP targets RAB11B-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	RAB11B, member RAS oncogene family
Calculated molecular weight	24 kDa
Observed molecular weight	24 kDa
GenBank accession number	NM_004218
Gene Symbol	RAB11B
Gene ID (NCBI)	9230
RRID	AB_10642010
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15907
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAB11B, also named as YPT3, belongs to the small GTPase superfamily and Rab family. RAB11B possesses GTPase activity. The antibody is specific to RAN11B. The antibody has no cross reaction to RAB11A.

Protocols

Product Specific Protocols
WB protocol for RAB11B-Specific antibody 19742-1-AP	Download protocol
IHC protocol for RAB11B-Specific antibody 19742-1-AP	Download protocol
IP protocol for RAB11B-Specific antibody 19742-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncotarget Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma. Authors - Hao Bo View Article
human	WB	J Exp Clin Cancer Res Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway. Authors - Yu Lian View Article
human	WB	Tumour Biol AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis. Authors - Zhaoyang Zeng View Article
canine,rat	WB,IHC	J Histochem Cytochem Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties. Authors - Hiroshi Gomi View Article
human	WB,IF	J Neurosci The Late Endosomal Pathway Regulates the Ciliary Targeting of Tetraspanin Protein Peripherin 2. Authors - Wataru Otsu View Article
human	WB,IP	Heliyon Host proteins interact with viral elements and affect the life cycle of highly pathogenic avian influenza A virus H7N9 Authors - Dong-Shan Yu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 19742-1-AP

WB analysis of human brain using 19742-1-AP

WB analysis of C6 using 19742-1-AP

WB analysis of PC-12 using 19742-1-AP

WB analysis of HEK-293 using 19742-1-AP

WB analysis of HeLa using 19742-1-AP

WB analysis of human testis using 19742-1-AP

WB analysis of mouse testis using 19742-1-AP

WB analysis of fetal human brain using 19742-1-AP

WB analysis of PC-12 using 19742-1-AP

IP experiment of mouse brain using 19742-1-AP

IHC staining of human lung using 19742-1-AP

IHC staining of human lung using 19742-1-AP

IHC staining of human placenta using 19742-1-AP

IHC staining of human placenta using 19742-1-AP

IHC staining of human brain using 19742-1-AP

IHC staining of human brain using 19742-1-AP

RAB11B-Specific Polyclonal antibody

RAB11B-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.

Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.

AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis.

Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties.

The Late Endosomal Pathway Regulates the Ciliary Targeting of Tetraspanin Protein Peripherin 2.

Host proteins interact with viral elements and affect the life cycle of highly pathogenic avian influenza A virus H7N9